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Application of Brassica napus-Isatis g monomer addition line in inhibiting novel coronavirus sars-cov-2

A technology of Brassica napus and coronavirus, applied in the fields of application, antiviral agents, respiratory diseases, etc., can solve the problems of unclear pathogenesis and virus-host interaction molecular mechanism, no virus treatment, etc., and achieve no resistance Drug properties, low cost, large output effect

Active Publication Date: 2021-10-29
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although many studies on SARS-CoV-2 have been published, the current understanding of this novel coronavirus is only the tip of the iceberg, and the pathogenesis of SARS-CoV-2 infection and the molecular mechanism of virus-host interaction are still very unclear There is no cure for this virus

Method used

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  • Application of Brassica napus-Isatis g monomer addition line in inhibiting novel coronavirus sars-cov-2
  • Application of Brassica napus-Isatis g monomer addition line in inhibiting novel coronavirus sars-cov-2
  • Application of Brassica napus-Isatis g monomer addition line in inhibiting novel coronavirus sars-cov-2

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Preparation of Brassica napus-Isatis indigo line extract:

[0051] The whole plants of different additional lines of Brassica napus-Isatis indica planted in the field were harvested, dried naturally in the shade or at low temperature, crushed, and passed through an 80-mesh sieve, and the collected powder was used for the preparation of crude extraction. The specific extraction steps are as follows:

[0052] 1) Add 100g of different additive powders and 1000mL of methanol into a 2000mL Erlenmeyer flask. Ultrasonic treatment at 55°C for 40-60 minutes, after filtration, collect the supernatant and filter residue respectively;

[0053] 2) Add 1000mL methanol to the filter residue in step 1 to continue ultrasonic treatment, cycle 5-6 times according to step 1, and combine all the supernatants obtained;

[0054] 3) Evaporate methanol from the supernatant in step 2 in a rotary evaporator at 50°C;

[0055] 4) Dissolve and evaporate the crude extract of methanol with single di...

Embodiment 2

[0060] Effects of extracts of different additional lines of Brassica napus-Isatis indica on the proliferation of SARS-CoV-2 virus at the cell level in vitro

[0061] 1. Cell culture

[0062] The frozen and revived Caco-2 cells were subcultured twice, and expanded with DMEM medium containing 10% fetal bovine serum and double antibodies (penicillin 100 U / ml, streptomycin 100 ug / ml).

[0063] 2. The cells were treated with the extract of Brassica napus-Isatis indigo line and infected with the new coronavirus WBP-1

[0064] 1) The Caco-2 cells in good growth state were digested and passaged, and the cell density was adjusted to 1×10 with cell culture medium. 5 / ml, 1ml per well was inoculated in a 12-well plate.

[0065] 2) When the cell growth monolayer density reaches 80%, add the extracts of different additional lines of Brassica napus-Isatis indigo to the Caco-2 cells in the 12-well plate, the extract concentration is 200mg / mL, and the working concentration is 400μg / mL mL. ...

Embodiment 3

[0078] Inhibitory effect of Brassica napus-Isatis indigo G monomer addition line on the proliferation of SARS-CoV-2 virus at the cell level in vitro:

[0079] According to the results of Example 2, it can be known that the G monomer addition system extract has a significant inhibitory effect on the new coronavirus when the working concentration is 4mg / mL, and Example 3 further confirms the anti-new coronavirus effect and the The inhibitory effect of different concentrations on the proliferation of the new coronavirus.

[0080] 1. Toxic effect of G monomer extract on cells

[0081] 1) Take well-grown Caco-2 cells for digestion and passage, and use cell growth medium (DMEM medium + 10% fetal bovine serum + double antibody) to adjust the cell density to 2 × 10 6 / ml, inoculate 96-well plate, inoculate 100μl per well;

[0082] 2) Add 100 μl G monomer extracts of different concentrations prepared with culture medium (DMEM medium + 10% serum + double antibody) to each well, and mi...

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Abstract

The invention belongs to the field of biotechnology, and discloses the application of Brassica napus-Isatis indigo G monomer addition system in inhibiting novel coronavirus SARS-CoV-2. The present invention finds for the first time that the extract of Brassica napus-Isatis indigo G monomer addition line has significant anti-SARS-CoV-2 virus effect on the cell infection model, and has no toxic side effects on cells. The additional line of Brassica napus-Isatis indica has the advantages of large-scale planting, which can provide sufficient raw materials for the production and extraction of anti-SARS-CoV-2 preparations, with low cost and large output, and can be used as anti-virus medicinal vegetables, feed, etc. It is developed and utilized as a health food for humans and animals to protect the health of animals and humans.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to the application of Brassica napus-Isatis indica G monomer addition line in inhibiting novel coronavirus SARS-CoV-2 Background technique [0002] Novel coronavirus disease 19 (COVID-19) is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). However, so far, there is no recognized effective treatment for new coronary pneumonia or anti-SARS-CoV-2 virus method. Therefore, it is of great significance to study and develop new anti-SARS-CoV-2 virus preparations or drugs. [0003] The Brassica napus-Isatis indigo addition line (abbreviated as Radix Radix Rapeseed or Youban) is an addition line obtained by hybridizing somatic cells of Brassica napus and Isatis indigo. Rapeseed has multiple functions and uses. The leaves and flower stalks can be used for vegetables, the seeds can be used for oil extraction, the rapeseed meal and green fodder can be used for animals, and th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/14A61P31/14A61P11/00A61K36/31
CPCA23V2002/00A61K36/31A61K36/315A61K49/0008A23K10/30A23L33/105A61P11/00A61P31/14A61K2300/00A23V2200/30A23V2250/21
Inventor 金梅林回显锋杨丽李再云黄坤龚文孝邹忠张宇飞钟鸣杜雪竹孙小美盛锋
Owner HUAZHONG AGRI UNIV
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