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A kind of separation method of banana anthracnose bacteria

A technology of banana anthracnose and anthracnose bacteria, applied in the field of plant pathology, can solve the problems of time-consuming and labor-intensive, affecting the efficiency and effect of separation work, and achieve the effect of improving efficiency and simple separation operation

Active Publication Date: 2022-06-17
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This type of method needs to carry out the spore quantification and dilution operation process of the spore liquid, which is time-consuming and labor-intensive, and it is easy to be contaminated by bacteria, which will affect the efficiency and effect of the separation work.

Method used

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  • A kind of separation method of banana anthracnose bacteria
  • A kind of separation method of banana anthracnose bacteria
  • A kind of separation method of banana anthracnose bacteria

Examples

Experimental program
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Effect test

preparation example Construction

[0035] 2. Preparation of Spore Discrete Filaments

[0036] Take out the pipette tip of a 1000μl ordinary pipette, put the tip of the pipette tip on the flame and burn, and the tip of the pipette tip will melt where the fire is. Clamp a bit of the melted tip and stretch it gently until a hair-like filament is formed. After cooling, cut off the excess filament, and keep the 3cm long filament attached to the tip to form a filament. The spore discrete filament used for the isolation of banana anthracnose spores, the discrete filament tool includes the sliding filament a and the stalk b part, such as figure 1 As shown, the two parts are naturally connected into one, and the key technical role is to slide silk a. The sliding wire a is small, soft and elastic.

[0037] When separating different specimens for use, first wipe the sliding wire a with a 75% alcohol cotton ball for surface disinfection, or repeat the previous operation and then burn and stretch a new sliding wire.

[0...

Embodiment 1

[0059] Using the method for separating banana anthracnose according to the present invention, the banana anthracnose of standard sample 1 of banana anthracnose is separated, and the isolation and culture results are as follows: Image 6 shown.

Embodiment 2

[0061] The banana anthracnose pathogen of banana anthracnose standard sample 2 is separated by the method for separating banana anthracnose according to the present invention, and the isolation and culture results are as follows: Figure 7 shown.

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Abstract

The invention discloses a method for isolating banana anthracnose bacteria. In this method, a special spore discrete silk is used to directly separate a single conidia from a standard sample of banana anthracnose, and culture it to form a strain of banana anthracnose with a single genetic composition. The implementation steps of isolation culture are as follows: 1) preparation of banana anthracnose standard sample; 2) preparation of spore discrete silk; 3) preparation of spore discrete plate; 4) separation of conidia; 5) small squares loaded with single spores 6) the formation of single spore isolated strains. The present invention has the following advantages: 1) the key utensils for separating spores are simple and easy to make, and the separation operation is simple and easy; 2) the separation and acquisition of monospore strains can be done at one time; 3) the separation work consumes less labor and time; 4) can Significantly improve the work efficiency and the reliability of the isolation results of banana anthracnose bacteria isolation.

Description

technical field [0001] The present invention relates to plant pathology techniques. Specifically, it is a method for separating banana anthracnose. Background technique [0002] Banana anthracnose is a major disease of banana industry in my country, which is caused by the infection of banana anthracnose (Colletotrichum musae). The biological research on banana anthracnose is an important basis for the prevention and control of banana anthracnose. The premise of carrying out biological research on banana anthracnose is to have banana anthracnose material. General laboratories often need to isolate and obtain banana anthracnose from disease standard samples. [0003] The genetic composition of banana anthracnose populations is complex, and the biological characteristics and pathogenicity of different strains are often quite different. Normal research usually requires the acquisition of banana anthracnose material with a single genetic composition, or a monoclonal strain ma...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12N1/02C12N3/00C12R1/645
CPCC12N1/14C12N1/02C12N3/00
Inventor 张容博张君成王忠文
Owner GUANGXI UNIV
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