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Industrialized tissue culture and rapid propagation method for astragalus membranaceus

A technology for tissue culture and rapid propagation of Astragalus membranaceus, which is applied in horticultural methods, botanical equipment and methods, climate change adaptation, etc., can solve problems such as endangered and difficult to obtain Astragalus membranaceus seedlings, etc., to meet production needs Effect

Active Publication Date: 2021-07-23
宁夏农林科学院农业生物技术研究中心
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Astragalus membranaceus is the top grade of Astragalus membranaceus, which has the functions of invigorating qi and raising yang, strengthening the surface and antiperspirant, diuresis and detumescence; it is a traditional Chinese medicinal material commonly used by doctors in the past dynasties, and the market demand is huge. It is on the verge of extinction. At present, the artificially cultivated Astragalus membranaceus is mostly mixed with Astragalus membranaceus, and it is difficult to obtain pure Astragalus membranaceus seedlings. Therefore, it is necessary to cultivate non-separating traits and consistent growth through tissue culture rapid propagation and industrial seedling technology. , Pure quality Astragalus membranaceus seedlings
At present, there are no reports on tissue culture, rapid propagation and industrial seedling cultivation of Astragalus membranaceus

Method used

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  • Industrialized tissue culture and rapid propagation method for astragalus membranaceus
  • Industrialized tissue culture and rapid propagation method for astragalus membranaceus
  • Industrialized tissue culture and rapid propagation method for astragalus membranaceus

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Embodiment 1

[0034] One, the acquisition of test-tube plantlets of Astragalus membranaceus, the specific steps are as follows:

[0035] 1. Explant selection and treatment

[0036] In late May, the wild Astragalus membranaceus has just grown new shoots of 10-15 cm. Select a healthy and fine individual plant, cut off the new shoots, wrap them in wet newspaper, put them in a sampling refrigerated incubator, and bring them back for tissue culture as soon as possible. room for pretreatment.

[0037] Take out the twigs of Astragalus membranaceus, wash the surface with running water for 0.5h, wash the twigs and leaves with a soft brush dipped in detergent water, rinse them with clean water, and divide them into 4-5cm segments with leaves.

[0038] 2. Explant disinfection:

[0039] In the ultra-clean workbench, put the divided explants into a sterile glass bottle with a cover, add 2% sodium hypochlorite to submerge the explants, drop 1-2 drops of Tween 20, and shake it by hand continuously For ...

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Abstract

The invention provides an industrialized tissue culture and rapid propagation method for astragalus membranacus, and relates to the technical field of tissue culture and rapid propagation of traditional Chinese medicines. Through callus induction, callus proliferation and differentiation culture and rooting culture, a large number of pure astragalus membranacus seedlings can be obtained all the year round. The proliferation coefficient of the astragalus membranaceus cluster buds obtained by the industrialized tissue culture and rapid propagation method reaches 13-15 times, the cluster buds are robust, the cluster buds are easy to root after being inoculated to an open rooting culture medium, the rooting rate reaches 90% or above, the later transplanting survival rate is 90% or above, and high-quality astragalus membranaceus seedlings are obtained. The industrialized tissue culture and rapid propagation method for astragalus membranacus is not limited by time and space, factory cultivation can be achieved all the year round, and production requirements are met.

Description

technical field [0001] The invention belongs to the technical field of tissue culture and rapid propagation of traditional Chinese medicine, and in particular relates to a method for industrialized tissue culture and rapid propagation of Astragalus membranaceus. Background technique [0002] Astragalus membrane pod (Astragalus membranaceus (fisch.) Bunge) is a perennial herbaceous plant of the leguminous family, a national third-level protected plant, and is a genuine product of Astragalus membranaceus in the Pharmacopoeia. It is produced in Heilongjiang, Jilin, Liaoning, Inner Mongolia, Hebei, Shanxi, Shaanxi, Ningxia, Gansu , Qinghai, Xinjiang, Shandong and Sichuan. It grows on the edge of the forest or under the shrubs and sparse forests. ) and Huayuan (Yao County, Shaanxi) are also good." The Liupanshan area of ​​Ningxia is the main producing area of ​​"Yuanzhou Astragalus", and the local people have a long history of artificial domestication and cultivation of wild Ast...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008Y02P60/40
Inventor 郭生虎陈虞超李明朱永兴关雅静张茜石磊刘华
Owner 宁夏农林科学院农业生物技术研究中心
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