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Tissue culture propagation method for Hainan dendrobe

A technology of tissue culture of Dendrobium hainan, applied in the field of plant propagation, can solve the problems of long time required, no germination ability, low reproduction rate, etc., and achieve the effect of realizing large-scale production, ensuring the quality of seedlings, and ensuring the multiplication coefficient

Inactive Publication Date: 2015-11-11
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, Hainan Dendrobium has been artificially cultivated, mainly through division or cuttings, but it takes a long time and the reproduction rate is low, so it is difficult to achieve large-scale cultivation in production.
Although Dendrobium hainan also has seeds, the seeds do not have endosperm and have no germination ability. Only under the symbiosis of fungi can the germination and growth of seeds be promoted, and the germination rate is very low, and the production needs cannot be met through seed breeding.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] An example of the tissue culture rapid propagation method of Dendrobium hainan of the present invention comprises the following steps:

[0015] (1) Selection and disinfection of explants: take the newly germinated tender shoots of the healthy plants of Dendrobium hainan as explants, after peeling off the outer wrapping leaves, soak with 2% (v / v) detergent aqueous solution for 5min, Rinse the thread with tap water for 15-30 minutes, add 2-3 drops of Tween-20 to 100 ml of 0.1% (v / v) mercury liter for disinfection for 8-10 minutes, rinse with sterile water for 3-5 times, and finally remove the surface with sterile filter paper Moisture to obtain explants, wherein the sterile water is distilled water sterilized by high pressure;

[0016] (2) The first generation of explants is induced to obtain sterile test-tube plantlets: the explants obtained in step (1) are placed under a dissecting microscope, the shoot tip meristem of 0.2-0.3 mm is stripped off, and inoculated into MS ...

Embodiment 2

[0019] Another example of the tissue culture rapid propagation method of Dendrobium hainan of the present invention comprises the following steps:

[0020] (1) Selection and disinfection of explants: take the newly germinated tender shoots of the healthy plants of Dendrobium hainan as explants, after peeling off the outer wrapping leaves, soak with 2% (v / v) detergent aqueous solution for 5min, Rinse the thread with tap water for 15-30 minutes, add 2-3 drops of Tween-20 to 100 ml of 0.1% (v / v) mercury liter for disinfection for 8-10 minutes, rinse with sterile water for 3-5 times, and finally remove the surface with sterile filter paper Moisture to obtain explants, wherein the sterile water is distilled water sterilized by high pressure;

[0021] (2) The first generation of explants is induced to obtain sterile test-tube plantlets: the explants obtained in step (1) are placed under a dissecting microscope, the shoot tip meristem of 0.2-0.3 mm is stripped off, and inoculated int...

Embodiment 3

[0024] Another example of the tissue culture rapid propagation method of Dendrobium hainan of the present invention comprises the following steps:

[0025] (1) Selection and disinfection of explants: take the newly germinated tender shoots of the healthy plants of Dendrobium hainan as explants, after peeling off the outer wrapping leaves, soak with 2% (v / v) detergent aqueous solution for 5min, Rinse the thread with tap water for 15-30 minutes, add 2-3 drops of Tween-20 to 100 ml of 0.1% (v / v) mercury liter for disinfection for 8-10 minutes, rinse with sterile water for 3-5 times, and finally remove the surface with sterile filter paper Moisture to obtain explants, wherein the sterile water is distilled water sterilized by high pressure;

[0026] (2) The first generation of explants is induced to obtain sterile test-tube plantlets: the explants obtained in step (1) are placed under a dissecting microscope, the shoot tip meristem of 0.2-0.3 mm is stripped off, and inoculated int...

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Abstract

The invention provides a tissue culture propagation method for Hainan dendrobe. The tissue culture propagation method comprises the following steps: (1) taking newly-sprouted tender buds of healthy plants of the Hainan dendrobe as explants and disinfecting; (2) putting the disinfected explants into an MS basic culture medium to be induced to obtain sterile test tube seedlings; and (3) putting the sterile test tube seedlings into an MS propagation culture medium to perform rapid propagation culture of the test tube seedlings to obtain cluster buds. With the adoption of the culture method provided by the invention, the growth coefficient of the obtained cluster buds of the Hainan dendrobe can be increased by 15-20 times, high-quality Hainan dendrobe germchits with a high survival rate can be provided in short time, and a large-scale seedling growing problem of the Hainan dendrobe is effectively solved.

Description

technical field [0001] The invention relates to a method for plant propagation, in particular to a method for tissue culture and propagation of Dendrobium hainanensis. Background technique [0002] Dendrobium hainanense Rolfe is a perennial plant of the genus Dendrobium in the family Orchidaceae, which grows on tree trunks in mountainous broad-leaved forests at an altitude of 1000-1700 meters. Distributed in Hong Kong, China, Hainan, Vietnam, and Thailand, it has high horticultural value. [0003] At present, Hainan Dendrobium has been artificially cultivated, mainly by division or cuttings, but it takes a long time and the reproduction rate is low, making it difficult to achieve large-scale cultivation in production. Although Hainan Dendrobium also has seeds, the seeds do not have endosperm and have no germination ability. Only under the symbiosis of fungi can the germination and growth of seeds be promoted, and the germination rate is very low, and the production needs ca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 韦坤华彭玉德刘凡吕惠珍李林轩缪剑华李翠王晓峰
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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