Tissue Culture and Rapid Propagation Method of Orchid chinensis
A technique for straight-lipped orchid and tissue culture, which is applied in the field of tissue culture and plant reproduction to achieve the effects of ensuring the multiplication coefficient and seedling quality, improving the seedling rate, and cooperating closely.
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example 1
[0030] A method of tissue culture and rapid propagation of straight lip curly orchids includes the following steps:
[0031] Step 1. Take the newly germinated buds of C. serrata as explants and place them in the first culture medium to obtain sterile test tube plantlets; wherein, the first culture medium includes: Huabao No. 1 medium , 0.3mg / L methionazine, 1.5mg / L naphthaleneacetic acid, 1.5mg / L polyvinylpyrrolidone, 25g / L sucrose and 4.5g / L agar;
[0032] Step 2. Place the sterile test-tube plantlet obtained in step 1 in a second medium for culturing to obtain a clumping bud of the test-tube plantlet; wherein, the second medium includes: Huabao No. 1 medium, 0.5 mg / L A Thiadazine, 1.0mg / L naphthaleneacetic acid, 0.2mg / L kinetin, 1.5mg / L polyvinylpyrrolidone, 25g / L sucrose and 4.5g / L agar;
[0033] Step 3. Place the test-tube clumping buds obtained in step 2 in the third medium to obtain robust seedlings; wherein, the third medium includes: MS, 0.1 mg / L naphthalene acetic acid, 1.2...
example 2
[0047] A method of tissue culture and rapid propagation of straight lip curly orchids includes the following steps:
[0048] Step 1. Take the newly germinated buds of C. serrata as explants and place them in the first culture medium to obtain sterile test tube plantlets; wherein, the first culture medium includes: Huabao No. 1 medium , 0.7mg / L methionazine, 2.5mg / L naphthaleneacetic acid, 2.5mg / L polyvinylpyrrolidone, 35g / L sucrose and 5.5g / L agar;
[0049] Step 2. Place the sterile test-tube plantlet obtained in step 1 in a second medium for cultivation, to obtain a clumping bud of the test-tube plantlet; wherein, the second medium includes: Huabao No. 1 medium, 1.5 mg / L A Thiadazine, 2.5mg / L naphthaleneacetic acid, 0.5mg / L kinetin, 2.5mg / L polyvinylpyrrolidone, 35g / L sucrose and 5.5g / L agar;
[0050] Step 3. Place the test-tube clumping buds obtained in step 2 into the third medium to obtain robust seedlings; wherein, the third medium includes: MS, 0.3 mg / L naphthalene acetic acid...
example 3
[0064] A method of tissue culture and rapid propagation of straight lip curly orchids includes the following steps:
[0065] Step 1. Take the newly germinated buds of C. serrata as explants and place them in the first culture medium to obtain sterile test tube plantlets; wherein, the first culture medium includes: Huabao No. 1 medium , 0.5mg / L methionazine, 2mg / L naphthaleneacetic acid, 2mg / L polyvinylpyrrolidone, 30g / L sucrose and 5g / L agar;
[0066] Step 2. Place the sterile test tube plantlet obtained in step 1 in a second medium for cultivation to obtain a cluster of test tube plantlets; wherein the second medium includes: Huabao No. 1 medium, 0.5-1.5 mg / L Thioridazine, 1.0-2.5mg / L naphthaleneacetic acid, 0.2-0.5mg / L kinetin, 1.5-2.5mg / L polyvinylpyrrolidone, 25-35g / L sucrose and 4.5-5.5g / L Agar
[0067] Step 3. Place the test-tube clumping buds obtained in step 2 in the third medium to obtain robust seedlings; wherein, the third medium includes: MS, 0.2 mg / L naphthalene acetic...
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