Two-step seedling formation method of daphniphyllum calycinum
A technology for planting maple and seedlings, applied in the field of plant tissue culture, can solve problems such as tissue culture of maple trees that have not been seen, and achieve the effects of ensuring the quality of seedlings, ensuring the multiplication coefficient, and realizing large-scale production.
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Embodiment 1
[0022] A two-step method for growing seedlings of maple leaves, comprising the following steps:
[0023] (1) Take the young leaves of the newly sprouted maple as explants, and put them in the ultra-clean workbench, cut the edge of the leaves with a scalpel, cut them into small pieces of 1 cm square, and place them in WPM for cultivation medium, cultivated for 35 days under the condition of culture temperature of 23°C, light intensity of 1500lux, and light time of 8h / day to obtain sterile test-tube plantlets; wherein the WPM medium contained 1.0mg / L brassinolide, 0.2 mg / L indolebutyric acid, 0.2mg / L kinetin, 1.0mg / L gibberellin, 2mg / L polyvinylpyrrolidone PVP, 30g / L sucrose and 5g / L agar, the pH value of the medium is 5.8;
[0024] (2) Rooting culture: Place the robust plants obtained in step (1) in 1 / 2MS rooting medium at a culture temperature of 23°C, a light intensity of 3000 lux, and a light time of 8 hours / day for 30 days to obtain complete Rooted shoots, wherein 1.0mg / L...
Embodiment 2
[0027] A two-step method for growing seedlings of maple leaves, comprising the following steps:
[0028] (1) Take the young leaves of the newly sprouted maple as explants, and put them in the ultra-clean workbench, cut the edge of the leaves with a scalpel, cut them into small pieces of 1 cm square, and place them in WPM for cultivation culture medium, at a culture temperature of 27°C, a light intensity of 1500 lux, and a light time of 10 hours / day to cultivate sterile test-tube plantlets for 35 days; wherein the WPM medium contains 1.5 mg / L of brassinolide, 0.2mg / L indolebutyric acid, 0.2mg / L kinetin, 0.5mg / L gibberellin, 2mg / L polyvinylpyrrolidone PVP, 30g / L sucrose and 5g / L agar, the pH of the medium The value is 5.8;
[0029] (2) Rooting culture: place the robust plants obtained in step (1) in 1 / 2MS rooting medium at a culture temperature of 27°C, with a light intensity of 3000 lux and a light time of 10 hours / day for 30 days to obtain complete Rooted shoots, wherein 1 / 2...
Embodiment 3
[0032] A two-step method for growing seedlings of maple leaves, comprising the following steps:
[0033] (1) Take the young leaves of the newly sprouted maple as explants, and put them in the ultra-clean workbench, cut the edge of the leaves with a scalpel, cut them into small pieces of 1 cm square, and place them in WPM for cultivation culture medium, at a culture temperature of 25°C, a light intensity of 1500 lux, and a light time of 9 hours / day, cultured for 35 days to obtain sterile test-tube plantlets; wherein the WPM medium contained 2.0 mg / L of brassinolide, 0.2mg / L indolebutyric acid, 0.5mg / L kinetin, 0.5mg / L gibberellin, 2mg / L polyvinylpyrrolidone PVP, 30g / L sucrose and 5g / L agar, the pH of the medium The value is 5.8;
[0034] (2) Rooting culture: place the strong plants obtained in step (1) in 1 / 2MS rooting medium at a culture temperature of 25°C, light intensity of 3000 lux, and light time of 9 hours / day under the condition of cultivating for 30 days to obtain com...
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