A kind of rapid propagation method of rabbit ear orchid leaf tissue culture

Abstract

The invention relates to a cymbidium lancifolium leaf tissue culture rapid propagation method comprising the following steps: (1) cymbidium lancifolium sterile test-tube seedling young leaves are adopted as explants, and are disinfected; (2) the disinfected explants are subjected to induction culture in an MS basic medium, such that sterile test-tube seedlings are obtained; (3) the sterile test-tube seedlings are subjected to rapid propagation culture in an MS propagation medium, such that cluster buds are obtained; (4) the cluster buds are subjected to seedling-strengthening culture in an MS seedling-strengthening medium, such that robust plants are obtained; (5) the robust plants are subjected to rooting culture in a 1 / 2 MS rooting medium, such that complete seedlings with roots are obtained; and (6) the complete seedlings with roots are subjected to seedling hardening for 4-7 days under an environment with a room temperature of 25 DEG C, and are transplanted into a sterilized coco coir medium. With the method provided by the invention, cymbidium lancifolium high-quality seedlings with high survival rate can be provided within a short time, and a problem of cymbidium lancifolium large-scale seedling raising is effectively solved.

Description

technical field [0001] The invention relates to a plant propagation method, in particular to a rapid propagation method of rabbit ear orchid leaf tissue culture. Background technique [0002] Cymbidium lancifolium (Cymbidium lancifolium), also known as broad-leaved orchid, is a semi-epipphytic plant of the orchid family first published in "Exotic Flora" by British scholar Hooke in 1823. It is distributed in Guangdong, Guangxi, Sichuan, and Yunnan. , Guizhou, China Taiwan and Tibet and other provinces, Japan, Vietnam, Nepal, India and Malaysia are also distributed. [0003] Rabbit ear orchid is a national first-level key protected wild plant. It is one of the Chinese orchids with great ornamental value, with peculiar leaf shape, elegant flowers, quiet fragrance and green and white flower color. In addition, the whole herb can also be used as medicine. It can be harvested throughout the year, washed, cut into sections and used fresh or dried. Carbuncles, sores, furuncles, r...

AI Technical Summary

Problems solved by technology

Therefore, at present, rabbit ear orchids are mainly propagated through traditional ramets, and the reproduction coefficient is extremely low, which cannot meet the needs of large-scale artificial cultivation.

[0005] Although there are also a small number of research reports related to the tissue culture of rabbit ear orchids and their hybrid progeny, they mainly use shoot tips, (axillary) buds or rhizomes as explants, and use basic medium, plant growth regulators, and organic additives as explants. In addition, there are still many problems in general, such as: internal and external pollution caused by bacteria and fungi easily produced by explants, low regeneration ability of explant cells, and in the air. It is easy to turn brown or exhausted, long growth cycle, germination and synchronous rooting problems, etc., and the proliferation rate of tissue culture is relatively slow (Tian Yingcui, Yang Liuqing. Research on rapid propagation technology of rabbit ear orchid tissue culture. Jiangsu Agricultural Science. 2007 (02): 106 -108; Chen Yunxi, He Dandan, Liao Haoru, et al. Factors Affecting the Differentiation of Rhizomes and Buds of Molan × Rabbit Ear Orchid. China Agronomy Bulletin. 2010(09):65-69; Yin Jing, Xu Chen. Guolan Sterile Research progress of rapid propagation system. Anhui Agricultural Sciences. 2010(32):18071-18073.)