A kind of hard leaf orchid tissue culture breeding method
A technology of tissue culture and sclerophyllum, which is applied in the field of plant propagation, can solve the problems of inability to store nutrients, difficulty in renewing resources, and low natural reproduction rate, and achieve the effects of ensuring seedling quality, realizing large-scale production, and ensuring multiplication coefficient
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Embodiment 1
[0013] An example of the sclerophylla tissue culture breeding method of the present invention comprises the following steps:
[0014] (1) Selection and disinfection of explants: take the newly germinated shoots of healthy plants of Sclerophyllum sclerophyllum as explants, peel off the outer wrapping leaves, soak them in 2v / v% aqueous solution of detergent for 5min, and linearly Rinse with tap water for 15-30min, add 2-3 drops of Tween-20 to 100ml of 0.1v / v% mercuric chloride for disinfection for 8-10min, rinse with sterile water for 3-5 times, and finally use sterile filter paper to remove surface moisture to obtain the Implants, wherein the sterile water is distilled water sterilized by high temperature and high pressure;
[0015] (2) First-generation induction to obtain aseptic test-tube plantlets: the explants obtained in step (1) are placed under a dissecting microscope, and the shoot tip meristem of 0.2-0.3mm is stripped off, inoculated into MS induction medium, and cultu...
Embodiment 2
[0018] Another example of the sclerophylla tissue culture breeding method of the present invention comprises the following steps:
[0019] (1) Selection and disinfection of explants: take the newly germinated shoots of healthy plants of Sclerophyllum sclerophyllum as explants, peel off the outer wrapping leaves, soak them in 2v / v% aqueous solution of detergent for 5min, and linearly Rinse with tap water for 15-30min, add 2-3 drops of Tween-20 to 100ml of 0.1v / v% mercuric chloride for disinfection for 8-10min, rinse with sterile water for 3-5 times, and finally use sterile filter paper to remove surface moisture to obtain the Implants, wherein the sterile water is distilled water sterilized by high temperature and high pressure;
[0020] (2) First-generation induction to obtain aseptic test-tube plantlets: the explants obtained in step (1) are placed under a dissecting microscope, and the shoot tip meristem of 0.2-0.3mm is stripped off, inoculated into MS induction medium, and ...
Embodiment 3
[0023] Another example of the sclerophylla tissue culture breeding method of the present invention comprises the following steps:
[0024] (1) Selection and disinfection of explants: take the newly germinated shoots of healthy plants of Sclerophyllum sclerophyllum as explants, peel off the outer wrapping leaves, soak them in 2v / v% aqueous solution of detergent for 5min, and linearly Rinse with tap water for 15-30min, add 2-3 drops of Tween-20 to 100ml of 0.1v / v% mercuric chloride for disinfection for 8-10min, rinse with sterile water for 3-5 times, and finally use sterile filter paper to remove surface moisture to obtain the Implants, wherein the sterile water is distilled water sterilized by high temperature and high pressure;
[0025] (2) First-generation induction to obtain aseptic test-tube plantlets: the explants obtained in step (1) are placed under a dissecting microscope, and the shoot tip meristem of 0.2-0.3mm is stripped off, inoculated into MS induction medium, and ...
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