Rapid propagation method of wild betula platyphylla suk

Abstract

The invention provides a rapid propagation method of wild betula platyphylla suk. The rapid propagation method comprises the following steps: 1) obtainment of the stem explant of the wild betula platyphylla suk; 2) inducing culture of the stem explant of the wild betula platyphylla suk; 3) enrichment culture of clustered shoots of the wild betula platyphylla suk; 4) rooting culture of the wild betula platyphylla suk: at the first stage, firstly culturing single shoots cut from the clustered shoots in a first rooting culture medium, putting the single shoots under 2800-3000Lux and illuminating the single shoots for 20 hours everyday to culture the single shoots until the single shoots root for 8-10 days; at the second stage, transferring the single shoots to a second rooting culture medium to be cultured for 20-30 days until good root systems grow; 5) hardening and transplanting of wild betula platyphylla suk tissue culture seedlings: carrying out hardening under normal temperature for 7-10 days and then transplanting the seedlings to a substrate to be cultured for 30-35 days, thus obtaining rapid propagation seedlings of the wild betula platyphylla suk; 6) transplanting: transplanting the seedlings to planting sites. The problems that the wild betula platyphylla suk is difficult in tissue culture and rooting and has low rooting rate are solved by adopting the rapid propagation method.

Description

technical field [0001] The invention relates to a rapid propagation method of wild white birch. Background technique [0002] White birch (Bettlla platyphylla suk), belonging to Betaceae Birch, deciduous tree, up to 20-25m in height, 50cm in diameter at breast height, white, smooth, papery layered peeling off; twigs brown reddish brown, leaves triangular oval , apex acuminate, base truncated, nutlets flat, membranous wings on both sides, flowering in May-June, fruit ripening in August. [0003] White birch is produced in the Greater Khingan Mountains, the Lesser Khingan Mountains, the Changbai Mountains in Northeast my country, the Altai Mountains in the North China and the Altai Mountains in the Northwest; it is distributed in the Greater Khingan Mountains, the Lesser Khingan Mountains, and the Changbai Mountains in Northeast China, Shanxi, Hunan, and Hubei in North China, and Inner Mongolia, Ningxia, Shaanxi, Qinghai, and Tibet in the Northwest. , Sichuan in the southwest,...

AI Technical Summary

Problems solved by technology

At present, white birch has been listed as a national second-class protected plant and a key plant protection resource. Moreover, white birch mainly reproduces by seeds and the reproduction speed is slow, which is difficult to meet market demand.

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