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Venous indwelling needle based on liquid metal and electrostatic spinning modification and preparation method thereof

A venous indwelling needle and liquid metal technology, applied in the field of medical devices, can solve the problems of skin tissue damage and low capture efficiency, achieve high sensitivity and specificity, good biological safety, and increase capture efficiency

Active Publication Date: 2021-07-16
SOUTH UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the previous research results also show that the capture efficiency of purely using indwelling needles is very low, only about 2%. At the same time, light-controlled therapy may cause damage to normal skin tissue

Method used

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  • Venous indwelling needle based on liquid metal and electrostatic spinning modification and preparation method thereof
  • Venous indwelling needle based on liquid metal and electrostatic spinning modification and preparation method thereof
  • Venous indwelling needle based on liquid metal and electrostatic spinning modification and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] This embodiment provides a kind of venous indwelling needle based on liquid metal and electrospinning modification and its preparation method. The design route of the venous indwelling needle is as follows figure 1 Shown:

[0069] First, spray liquid metal + / - electrodes on the designed area of ​​the indwelling needle surface, then coat nanofibers on the surface of the indwelling needle hose / electrode, and then modify the antibody on the surface of the nanofibers to obtain the liquid metal and electrode based on the present invention. Intravenous indwelling needle modified by electrospinning;

[0070] Further, the intravenous indwelling needle is inserted into the tail vein of the mouse to capture CTCs, and after the antibody captures the CTCs cells, the cells are killed by irreversible electroporation.

[0071] The specific preparation method is as follows:

[0072] (1) Liquid metal and polymer functionalization

[0073] a. Add 3g gallium indium alloy to a 5mL round...

Embodiment 2

[0093] This example investigates the biological safety of the intravenous indwelling needle at the cellular level.

[0094] In this example, the liver cancer cell lines HepG2, HUVECs and HeLa were selected as the research objects to investigate the biocompatibility of the intravenous indwelling needle catheter.

[0095] Inoculate and culture the above 3 kinds of cells in 96-well plate, after the cells grow overnight, perform the following operations in the well plate respectively (group 1: negative control; group 2: add a 5mm long catheter / MPC; group 3: add A 5mm long catheter / MPC / electrospinning).

[0096]After 24 hours of treatment, perform AO / PI fluorescence staining and use CCK-8 reagent for cell viability detection;

[0097] The result is as Figure 7 and Figure 8 As shown, the obtained intravenous indwelling has little effect on the activity of HepG2, HUVECs and HeLa cells, especially the effect on HepG2 is basically negligible, indicating that the intravenous indwel...

Embodiment 3

[0099] In this example, the function of enriching and killing CTCs of a dual-functional catheter was studied in a peripheral blood closed circulation simulation device.

[0100] (1) Selection and verification of EpCAM positive and negative expression cell models

[0101] Find the expression level of EpCAM in different cells from the website https: / / www.proteinatlas.org / ENSG00000119888-EPCAM / cell, and finally select HepG2 and HeLa cells as the cell models for positive and negative expression of EpCAM.

[0102] The expression of EpCAM on the surface of the two cells was further verified by flow cytometry, immunofluorescence and western blot;

[0103] (2) Construction of peripheral blood circulation simulation device

[0104] Use a peristaltic pump and a hose with an inner diameter of 2.5mm to build a peripheral blood circulation simulation device. The length of the inlet tube is 30cm, the length of the outlet tube is 15cm, and the hose is sealed with 3% BSA for 30min;

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Abstract

The invention provides a venous indwelling needle based on liquid metal and electrostatic spinning modification and a preparation method of the venous indwelling needle, and belongs to the technical field of medical instruments. The surface of a catheter of the venous indwelling needle comprises an electrode and a coating layer; the electrode comprises liquid metal and a high-molecular polymer; the coating layer coats a catheter of the venous indwelling needle and an electrode on the surface of the catheter through an electrostatic spinning method, and the surface of the coating layer is modified with a capture antibody of circulating tumor cells. The electrostatic spinning coating of the venous indwelling needle increases the capturing efficiency of circulating tumor cells, and irreversible electroporation killing is achieved through the liquid metal electrode. The invention provides a new in-vivo clearing strategy for clearing the circulating tumor cells by taking a liquid metal and electrostatic spinning functionalized venous indwelling needle as a carrier, enriching and then carrying out irreversible electroporation killing.

Description

technical field [0001] The invention belongs to the technical field of medical devices, and in particular relates to a venous indwelling needle and a preparation method thereof, in particular to a venous indwelling needle based on liquid metal and electrospinning modification and a preparation method thereof. Background technique [0002] Circulating tumor cells (CTCs) are a type of tumor cells that are released from solid tumors or metastases into the peripheral circulation spontaneously or due to diagnostic and therapeutic procedures. Studies have shown that circulating tumor cells are closely related to cancer recurrence and metastasis. In patients with metastatic breast cancer and metastatic colon cancer, the number of CTCs has become an important predictor for evaluating progression-free survival and overall survival. Therefore, reducing or eliminating CTCs in peripheral blood is of great significance for reducing the chance of postoperative hematogenous metastasis. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61M5/158C07K16/00
CPCA61M5/158C07K16/00A61M2207/00
Inventor 蒋兴宇王斗
Owner SOUTH UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA
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