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New application of 15S-HEPE in enhancing T cell mediated tumor immunotherapy

A 15S-HEPE, immunotherapy technology, applied in the field of medicine and immunotherapy, can solve the problem that the treatment method is difficult to achieve the desired effect

Inactive Publication Date: 2021-07-16
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these treatments are less effective due to diverse resistance mechanisms

Method used

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  • New application of 15S-HEPE in enhancing T cell mediated tumor immunotherapy
  • New application of 15S-HEPE in enhancing T cell mediated tumor immunotherapy
  • New application of 15S-HEPE in enhancing T cell mediated tumor immunotherapy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Effect of 15S-HEPE on the growth of B16-F1 cells cultured in vitro

[0019] 1. Experimental materials

[0020] Cell line: mouse melanoma cell line B16-F1

[0021] Reagent consumables: RPMI-1640 culture medium (Wiesent), trypsin cell digestion solution (Biyuntian), fetal bovine serum (BI), 96-well plate (NEST), MTT (Biyuntian), DMSO (Shanghai Sinopharm)

[0022] Instruments: cell incubator, ultra-clean bench, microplate reader

[0023] 2. Experimental method:

[0024] Take the B16 cells in the logarithmic growth phase, digest them with 0.25% trypsin, collect the cells, count the cells, prepare a single-cell suspension with a concentration of 20,000 cells / ml and culture them in a 96-well plate, with 2,000 cells per well (100 μL), at 37°C 5%CO 2 For culture, add 1 μL sterile physiological saline containing 0.1% ethanol to the solvent control group (Vehicle), add 1 μL sterile physiological saline containing corresponding concentrations of 15S-HEPE and 0.1% ethanol to th...

Embodiment 2

[0028] Effect of 15S-HEPE on the growth of B16 melanoma inoculated in nude mice

[0029] 1. Experimental materials

[0030] Cell line: mouse melanoma cell line B16-F1

[0031] Experimental animals: SPF grade nude mice

[0032] Reagent consumables: RPMI-1640 culture medium (Wiesent), trypsin cell digestion solution (Beiyuntian), fetal bovine serum (BI), 10cm petri dish (NEST), 1mL disposable sterile syringe, 100μL microsyringe,

[0033] Instruments: cell incubator, ultra-clean bench, SPF animal facilities

[0034] 2. Experimental method

[0035] B16 cells were seeded in 10cm culture dishes at 37°C with 5% CO 2 Cultivate for 24-36 hours to keep the cells in the logarithmic growth phase; digest the cells with trypsin cell digestion solution, collect the cells, count the cells, and prepare 3.33×10 6 Single cell suspension at a concentration of 1 / ml; each nude mouse was inoculated on the outside of the right hind leg, and 30 μL of single cell suspension was injected intraderma...

Embodiment 3

[0039] Effect of 15S-HEPE on the growth of B16 melanoma inoculated in C57BL / 6J mice

[0040] 1. Experimental materials

[0041] Cell line: mouse melanoma cell line B16-F1

[0042] Experimental animals: SPF grade C57BL / 6J mice

[0043] Reagent consumables: RPMI-1640 culture medium (Wiesent), trypsin cell digestion solution (Beiyuntian), fetal bovine serum (BI), 10cm petri dish (NEST), 1mL disposable sterile syringe, 100μL microsyringe.

[0044] Instruments: cell incubator, ultra-clean bench, SPF animal facilities

[0045] 2. Experimental method

[0046] B16 cells were seeded in 10cm culture dishes at 37°C with 5% CO 2 Cultivate for 24-36 hours to keep the cells in the logarithmic growth phase; digest the cells with trypsin cell digestion solution, collect the cells, count the cells, and prepare 3.33×10 6 single cell suspension at a concentration of 1 / ml; each C57BL / 6J mouse was inoculated on the outside of the right hind leg, and 30 μL of single cell suspension was injecte...

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Abstract

The invention discloses a novel application of a lipid derivative 15S-HEPE as a T cell mediated anti-tumor immune activator in treatment of melanoma. The invention finds that the 15S-HEPE effectively inhibits the growth of melanin tumors by improving the infiltration and activation level of T cells in the melanin tumors. The anti-tumor activity of the 15S-HEPE provided by the invention can be used for preparing anti-melanin tumor immunotherapy drugs.

Description

technical field [0001] The invention belongs to the technical field of medicine and immunotherapy, and specifically relates to a new application of a fatty acid derivative 15S-HEPE produced by polyunsaturated fatty acid EPA catalyzed by 15-lipoxygenase, that is, 15S-HEPE is used as T cell-mediated anti Tumor immune activator, used for immunotherapy of tumors. [0002] technical background [0003] Melanoma is the most malignant skin-related tumor, and the incidence of melanoma has been on the rise in the past few decades. Melanoma accounts for the majority of deaths from skin-related neoplasms. Based on the different characteristics of melanoma, such as the location of occurrence, genetic background and mutation type, etc., a variety of treatment methods for melanoma including surgery, radiotherapy, chemotherapy and targeted therapy have been developed, and certain therapeutic effects have been achieved. Nevertheless, these treatments are less effective due to diverse resis...

Claims

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Application Information

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IPC IPC(8): A61K31/202A61P35/00
CPCA61K31/202A61P35/00
Inventor 沈萍萍赵敬发章文龙黄亚红
Owner NANJING UNIV
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