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Gas chromatography-mass spectrometry detection method for cuticle hydrocarbon of temperate bed bugs and tropical bed bugs

A technology of gas chromatography mass spectrometry and hydrocarbons, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve problems such as blurred boundaries, and achieve the effects of simple operation, high sensitivity, and strong specificity

Inactive Publication Date: 2021-07-13
CHINA ACADEMY OF RAILWAY SCI CORP LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Both species, temperate and tropical bedbugs, seem to thrive in mixed populations, and an increase in the prevalence of tropical bedbugs has even been found in Australia and the UK, suggesting that while species still have well-defined ecological niches, the boundaries are blurring

Method used

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  • Gas chromatography-mass spectrometry detection method for cuticle hydrocarbon of temperate bed bugs and tropical bed bugs
  • Gas chromatography-mass spectrometry detection method for cuticle hydrocarbon of temperate bed bugs and tropical bed bugs
  • Gas chromatography-mass spectrometry detection method for cuticle hydrocarbon of temperate bed bugs and tropical bed bugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Detection instrument: Agilent gas chromatography-mass spectrometer GC-MS; capillary column is non-polar column HP-5MS.

[0039] The gas chromatography-mass spectrometry detection method of temperate bedbug epidermis hydrocarbon of the present invention, its steps are as follows:

[0040] (1) Sample preparation (extraction of epidermal hydrocarbons)

[0041] Take a temperate bedbug, put it in a glass sample bottle, add 500 μL of n-hexane (just cover the insect body), extract for 10 minutes, take the supernatant, dry it with high-purity nitrogen, and then add 20 μL of n-hexane to re-dissolve the obtained extract Store at -20°C for later use;

[0042] (2) The sample to be tested is detected by gas chromatography-mass spectrometry

[0043] The gas chromatographic analysis adopts Agilent gas chromatography-mass spectrometry GC-MS and the capillary chromatographic column is a non-polar column HP-5MS, and the epidermal hydrocarbons of the sample to be tested are analyzed and...

Embodiment 2

[0051] Detection instrument: Agilent gas chromatography-mass spectrometer GC-MS, and the capillary column is a non-polar column HP-5MS.

[0052] The gas chromatography-mass spectrometry detection method of tropical bedbug cuticle hydrocarbon of the present invention, its steps are as follows:

[0053] (1) Sample preparation (extraction of epidermal hydrocarbons)

[0054] Take a tropical bedbug and place it in a glass sample bottle, add 500 μL of n-hexane (just cover the insect body), extract for 10 minutes, take the supernatant, dry it with high-purity nitrogen, then add 20 μL of n-hexane to redissolve, and place the extract in Reserve at -20°C;

[0055] (2) The sample to be tested is detected by gas chromatography-mass spectrometry

[0056]The gas chromatography analysis adopts Agilent gas chromatography-mass spectrometry instrument GC-MS, and the capillary column is a non-polar column HP-5MS, and the epidermal hydrocarbons of the sample to be tested are analyzed and identi...

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Abstract

The invention relates to a gas chromatography-mass spectrometry detection method for cuticle hydrocarbons of temperate bed bugs and tropical bed bugs, which belongs to the technical field of hydrocarbon detection and comprises the following steps: (1) sample preparation; (2) carrying out gas chromatography-mass spectrometry detection on the sample to be detected; and (3) detection and analysis: comparing the insect epidermis hydrocarbon mass spectrum obtained by analysis of the mass spectrometer with a standard mass spectrum provided by a standard mass spectrum library, and selecting a standard compound with a high matching degree as a preliminarily determined type or structure. The gas chromatography-mass spectrometry detection method for cuticle hydrocarbon of temperate bugs and tropical bugs has the characteristics of high specificity, simplicity and convenience in operation, high sensitivity, short sample analysis time and practicability.

Description

technical field [0001] The invention relates to a gas chromatography-mass spectrometry method for detecting bedbugs, in particular to a gas chromatography-mass spectrometry detection method for epidermis hydrocarbons of temperate bedbugs and tropical bedbugs, and belongs to the technical field of hydrocarbon detection. Background technique [0002] Bedbugs belong to the class Insecta, Hemiptera, and Bed Bugs, and are a class of extradermal parasites that specialize in blood-sucking. Among them, temperate bedbugs and tropical bedbugs are closely related to humans, and they mainly live by sucking human blood. They are one of the blood-sucking sanitary pests that are extremely harmful to human harassment. Most people experience redness, itching, and other symptoms of an immune response after being bitten by a bedbug. Bed bug infestation can cause anxiety and insomnia, and also has the potential to be a vector. [0003] Global bedbug breeding occurred in the 1930s and 1940s. I...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/30G01N30/72
CPCG01N30/02G01N30/06G01N30/30G01N30/72
Inventor 靖博彬
Owner CHINA ACADEMY OF RAILWAY SCI CORP LTD
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