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Sperm counting method in egg zona pellucida binding test

A technology that combines testing and sperm counting, applied in the field of sperm counting, can solve the problems of high requirements for testing personnel, reduce testing costs, and complicated operations, and achieve the effects of reducing experimental errors, easy operation, and not easy to miss.

Inactive Publication Date: 2021-07-13
PEKING UNIV THIRD HOSPITAL
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AI Technical Summary

Problems solved by technology

The method is easy to operate, and the reagents used are easy to obtain, which can significantly reduce the cost of the test, and solve the technical problems of its complicated operation and high requirements for testing personnel, which limits its clinical promotion

Method used

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  • Sperm counting method in egg zona pellucida binding test

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Human egg zona pellucida binding test

[0033] 1. Reagents and equipment:

[0034] (1) Fertilization medium: G-IVF PLUS medium, Vitrolife, Sweden

[0035] (2) Eosin Y, 0.5(w / v): Dissolve 0.5g of Eosin Y in 100ml of 0.9% NaCl;

[0036] (3) Sperm and oocytes

[0037] The discarded sperm after fertilization in the conventional IVF cycle of patients undergoing in vitro fertilization treatment in the Reproductive Center of Peking University Third Hospital were collected. Collect the ICSI treatment cycle of this reproductive center, and discard the eggs that have failed fertilization on the third day after fertilization, and exclude immature and degenerated eggs. The research protocol has been approved by the Reproductive Medicine Ethics Committee of our hospital.

[0038] (4) G-MOPS PLUS culture medium (Vitrolife, Sweden)

[0039] (5) CO 2 Incubator (Thermo, 3131, USA)

[0040] (6) Stereo microscope (NIKON, SMZ1000, Japan)

[0041] (7) Inverted microscope (...

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Abstract

The invention provides an optimized sperm counting method for an egg zona pellucida binding test. The method is easy and convenient to operate, used reagents are easy to obtain, the test cost can be remarkably reduced, and the technical problem that clinical popularization is limited due to the fact that operation is complex and the requirement for detection personnel is too high is solved.

Description

technical field [0001] The invention relates to an optimized zona pellucida binding experiment method, more specifically, the invention relates to a sperm counting method in the egg zona pellucida binding test. Background technique [0002] Infertility is defined as the failure of a couple to achieve a clinical pregnancy after normal and frequent unprotected intercourse for 12 months or more. In developed countries, the incidence of infertility is 5.0-8.0%, while in developing countries it is as high as 5.8-44.2%, of which infertility due to male factors accounts for 40-50%, and about 2% of the sperm parameters of pregnant men Not up to par. At present, the clinical laboratory diagnosis of male infertility is mainly based on the "WHO Human Semen Examination and Processing Laboratory Manual (5th Edition)", using computer-aided sperm analysis (CASA) for routine semen analysis. Combining computer processing technology and advanced image processing technology, through a compre...

Claims

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Application Information

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IPC IPC(8): C12Q1/06
CPCG01N33/5005
Inventor 廉颖刘平乔杰王莹胡静李卓任秀莲李悦王亚朋王中伟
Owner PEKING UNIV THIRD HOSPITAL
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