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Angelica gigas Nakai extract as well as preparation method and application thereof

A technology of extracts and angelica, applied in the directions of pharmaceutical formulations, drug combinations, plant raw materials, etc., can solve problems such as poor prognosis, and achieve the effects of simple process, prevention and treatment of prostate cancer, and low manufacturing cost.

Active Publication Date: 2021-07-13
ZHUHAI COLLEGE OF JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The pathogenesis of prostate cancer is closely related to the androgen receptor. Androgen castration therapy is often used in clinical treatment. Although the disease progression can be controlled within a certain period of time for most patients, after 12-24 months of treatment, the disease will eventually develop. For castration-resistant prostate cancer, the prognosis is extremely poor, so there is still an urgent need for active research and development of a new drug for the treatment of prostate cancer

Method used

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  • Angelica gigas Nakai extract as well as preparation method and application thereof
  • Angelica gigas Nakai extract as well as preparation method and application thereof
  • Angelica gigas Nakai extract as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 The preparation method of Angelica sinensis extract KO.

[0021] A method for preparing an extract of Angelica sinensis, specifically comprising the following steps.

[0022] Step 1. Take soil Angelica medicinal material, add 95% v / v ethanol 8 times the weight of the medicinal material, reflux extraction for 2 hours, filter, add 5 times the amount of 95% ethanol to the filter residue, reflux extraction for 2 hours, filter, combine the filtrate, and concentrate under reduced pressure Extract 1 was obtained.

[0023] The extract 1 obtained in step 2 and step 1 is suspended by adding an appropriate amount of water to remove water-insoluble impurities such as resins and chlorophyll, and then use petroleum ether, ethyl acetate, and n-butanol organic solvents to carry out equal-volume extractions, twice each, After concentrating under reduced pressure, the extracts of the extracts were obtained. After removing the components of procurantin and angelica esters of pu...

Embodiment 2

[0024] Example 2 The Angelica sinensis extract provided by the present invention has significant preventive and therapeutic effects on the occurrence and growth of tumors in a transgenic mouse model of prostate cancer.

[0025] 1 Experimental materials.

[0026] 1.1 Experimental animals and feed.

[0027] The mothers of prostate cancer transgenic mice (TRAMP) were purchased from Jackson Laboratory, and the tails of offspring mice were sampled 3 weeks after birth for genetic testing. All mice were fed with AIN-93G feed and had free access to food and water.

[0028] 1.2 Experimental drugs.

[0029] The Angelica sinensis extract KO prepared in Example 1 was administered with ethanol-PEG400-Tween 80-5% glucose solution (EPTG ratio of 3:6:1:20) at a dosage of 80 mg / kg body weight (2mg / rat). ) to be prepared as a medicinal solution and administered by intragastric administration. Store at -20°C and pre-warm before oral administration.

[0030] 1.3 Experimental method.

[0031...

Embodiment 3

[0041] Example 3 The KO extract of Angelica sinensis of the present invention has a significant inhibitory effect on the tumor growth of human prostate cancer xenograft tumor mouse model.

[0042] 1 Experimental materials.

[0043] 1.1 Experimental animals and feed.

[0044] Immunodeficient mice were purchased from Jackson Laboratory, and all mice were fed with AIN-93G feed, free to eat and drink.

[0045] 1.2 Experimental drugs.

[0046] The Angelica sinensis extract KO prepared in Example 1 was administered with ethanol-PEG400-Tween 80-5% glucose solution (EPTG ratio 3:6:1:20) at a dose of 160mg / kg body weight (4mg / mouse) It is prepared into a liquid medicine and administered by intragastric administration. Store at -20°C and pre-warm before oral administration.

[0047] 1.3 Experimental method.

[0048] LNAR-luc cells were injected subcutaneously in the rear side of the body of double immunodeficient mice. Two days after inoculation, the mice were randomly divided into b...

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Abstract

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to an Angelica gigas Nakai extract for preventing and / or treating prostatic cancer as well as a preparation method and an application of the Angelica gigas Nakai extract. The main components of the Angelica gigas Nakai extract obtained by the preparation method provided by the invention are coumarin compounds and a small amount of flavonoids and phenolic acid compounds, and compared with the existing Angelica gigas Nakai extract preparation which mostly uses multiple organic solvents for extraction, macroporous resin elution, post-treatment and the like, the Angelica gigas Nakai extract is simple in process, low in preparation cost and high in yield, and the process is more suitable for large-scale industrial production of the Angelica gigas Nakai extract. Experiments prove that the Angelica gigas Nakai extract obtained by the preparation method disclosed by the invention has remarkable prevention and treatment effects on occurrence and growth of tumors of a prostatic cancer transgenic mouse model, and has a remarkable inhibition effect on tumor growth of a human prostatic cancer xenotransplantation tumor mouse model; and a wide prospect is provided for application of the compound in preparation of anti-prostatic cancer products.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine, and in particular relates to an extract KO of Angelica gigas Nakai for preventing and / or treating prostate cancer, a preparation method and application thereof. Background technique [0002] Soil Angelica, also known as Korean Angelica, Daduhuo, is a perennial herb belonging to the Umbelliferae Angelica Angelica gigas Nakai (AGN) dry root, in the Northeast of my country, Korean doctors use "Tanggui" as genuine Angelica among the people, thinking that it not only has the characteristics of Chinese traditional Chinese medicine Angelica ( Angelicae sinensis ) has the effect of promoting blood circulation and relieving pain, and also has the functions of clearing away heat and detoxification, expelling wind and dredging collaterals, etc. It can be used alone or in combination. [0003] Prostate cancer is one of the most common tumors of the male genitourinary system worldwide...

Claims

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Application Information

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IPC IPC(8): A61K36/232A61P35/00A61K125/00
CPCA61K36/232A61P35/00A61K2236/333A61K2236/39A61K2236/51A61K2236/55A61K2236/53
Inventor 吴威张会永张博
Owner ZHUHAI COLLEGE OF JILIN UNIV
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