Molecularly imprinted polymer electrode, preparation method and application of molecularly imprinted polymer electrode in electrochemical analysis of domoic acid

A technology of molecular imprinting and domoic acid, which is applied to the electrochemical variables of materials, material analysis through electromagnetic means, and material analysis. The effect of low requirements on the physical and chemical properties of the target and high-efficiency detection capabilities

Pending Publication Date: 2021-07-09
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Purpose of the invention: The present invention aims at the cumbersome detection process and difficult operation of domoic acid existing in the prior art, and provides a molecularly imprinted polymer electrode capable of accurately and quickly detecting domoic acid; also provides the above-mentioned molecularly imprinted polymer electrode Preparation method of polymer electrode and application of prepared molecularly imprinted polymer electrode in detection and analysis of domoic acid

Method used

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  • Molecularly imprinted polymer electrode, preparation method and application of molecularly imprinted polymer electrode in electrochemical analysis of domoic acid
  • Molecularly imprinted polymer electrode, preparation method and application of molecularly imprinted polymer electrode in electrochemical analysis of domoic acid
  • Molecularly imprinted polymer electrode, preparation method and application of molecularly imprinted polymer electrode in electrochemical analysis of domoic acid

Examples

Experimental program
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Effect test

Embodiment 1

[0040] Preparation of Polydopamine-Reduced Graphene Oxide / Polyacrylamide Molecularly Imprinted Polymer Electrode

[0041] (1) Put 60 mg of graphene oxide powder and 30 mg of dopamine hydrochloride in 60 mL of PBS with pH=8 and mix evenly, and stir at room temperature for 12 hours. After the reaction is completed, the mixture is centrifuged, washed, and vacuum-dried to obtain a PDA-rGO composite Material;

[0042] (2) Disperse PDA-rGO in deionized water to form a uniform 0.5 mg / mL suspension, take 5 μL of the suspension and drop-coat it on the SPE electrode, and dry it at room temperature;

[0043] (3) Dissolve acrylamide, methylenebisacrylamide and 1,3,5-pentanetricarboxylic acid in PBS at a concentration ratio of 2:1:1 (concentrations are 4mM, 2mM and 2mM, respectively), Take 5 μL of the solution and drop-coat it on the PDA-rGO modified SPE electrode, and use cyclic voltammetry to scan 20 circles in the potential range of -0.2 to 1.2 V at a scan rate of 100 mV / s to perform ele...

Embodiment 2

[0045] Preparation of Polydopamine-Reduced Graphene Oxide / Polyacrylamide Molecularly Imprinted Polymer Electrode

[0046] Change the acrylamide, methylene bisacrylamide and 1,3,5-pentanetricarboxylic acid in the step (3) of Example 1 to 0.5:1:1 according to the concentration ratio, and prepare the other conditions of the electrode unchanged, and obtain Polydopamine-reduced graphene oxide / polyacrylamide molecularly imprinted polymer.

Embodiment 3

[0048] Preparation of Polydopamine-Reduced Graphene Oxide / Polyacrylamide Molecularly Imprinted Polymer Electrode

[0049] Change the acrylamide, methylenebisacrylamide and 1,3,5-pentanetricarboxylic acid in the step (3) of Example 1 to 1:1:1 according to the concentration ratio, and prepare the other conditions of the electrode unchanged, and obtain Polydopamine-reduced graphene oxide / polyacrylamide molecularly imprinted polymer.

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Abstract

The invention discloses a molecularly imprinted polymer electrode, a preparation method and application of the molecularly imprinted polymer electrode in electrochemical analysis of domoic acid. The molecularly imprinted polymer electrode is a polydopamine-reduced graphene oxide/polyacrylamide molecularly imprinted polymer (PDA-rGO/PAM-MIP) electrode, graphene oxide is reduced and a composite material polydopamine-reduced graphene oxide is formed through a self-polymerization reaction of dopamine under an alkaline condition; the preparation method comprises the following steps of preparing a polydopamine-reduced graphene oxide modified screen-printed electrode by adopting a dispensing method, and preparing polyacrylamide through an electrochemical polymerization reaction to prepare a PDA-rGO/PAM-MIP electrode; according to the molecularly imprinted polymer electrode prepared by the invention, the domoic acid can be accurately and quickly detected.

Description

technical field [0001] The invention relates to a molecularly imprinted polymer electrode, a preparation method and its application in the electrochemical analysis of domoic acid, in particular to a polydopamine-reduced graphene oxide / polyacrylamide molecularly imprinted polymer electrode, a preparation method and Its application in the electrochemical analysis of domoic acid. Background technique [0002] Domoic acid is derived from the toxin-producing diatom Pseudorhomboides, which can be enriched in shellfish and transferred to senior consumers such as birds, marine mammals, and humans through the food chain. As an amnestic shellfish toxin, it is extremely toxic to the nerves and can cause loss of memory function, accompanied by symptoms such as dizziness, vomiting, abdominal pain and diarrhea, blurred vision, and confusion of thinking. So far, the discovery of toxin-producing diatoms has been reported in sea areas of various countries, and domoic acid has been detected ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N27/49
CPCG01N27/3277G01N27/49
Inventor 李曹龙姜孟楠唐俊彦王飞
Owner CHINA PHARM UNIV
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