Method for analyzing anti-osteoporosis action mechanism of ginseng-mulberry

A technology of anti-osteoporosis and mechanism of action, applied in the field of botanical drug pair research, can solve problems such as unreported

Pending Publication Date: 2021-06-29
CHANGCHUN UNIV OF CHINESE MEDICINE
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Problems solved by technology

In recent years, a large number of studies have shown that both ginseng and mulberry have the effect of treating osteoporosis, but at present, the research mainly focuses on the treatment of osteoporosis with a single herb, and the research on the treatment of osteoporosis after the combination of the two drugs has not been reported.

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  • Method for analyzing anti-osteoporosis action mechanism of ginseng-mulberry
  • Method for analyzing anti-osteoporosis action mechanism of ginseng-mulberry
  • Method for analyzing anti-osteoporosis action mechanism of ginseng-mulberry

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Embodiment Construction

[0030] A method for analyzing the anti-osteoporosis mechanism of ginseng-mulberry, including in vitro pharmacodynamic analysis, network pharmacology analysis, and molecular docking analysis;

[0031] specific,

[0032] In vitro pharmacodynamic analysis

[0033] Take ginseng single herb, mulberry single herb, and ginseng-mulberry combined medicine, add 10 times the amount of water and 8 times the amount of ethanol respectively, soak for 0.5 h, extract twice, each time for 1 h and 2 h, and combine the filtrates twice to obtain Ginseng water extract GW (abbreviated as GW), mulberry water extract MW (abbreviated as MW), ginseng alcohol extract GC (abbreviated as GC), mulberry alcohol extract MC (abbreviated as MC), ginseng and mulberry water extract compatibility GMW (abbreviated GMW), ginseng and mulberry alcohol extracts are compatible with GMC (GMC for short), concentrated under reduced pressure to make a dry paste, and stored in a refrigerator at 4°C for later use.

[0034] ...

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Abstract

The invention discloses a method for analyzing an anti-osteoporosis action mechanism of ginseng-mulberry, and belongs to the technical field of plant drug pair research. The method comprises the following steps: combining in-vitro pharmacodynamics with network pharmacology and a molecular docking technology to research the influence of single ginseng and mulberry and different polar components of the drug pair on the proliferation rate and the testosterone secretion of mouse leydig cells TM3 and the influence of the different polar components of the drug pair on the proliferation rate and the alkaline phosphatase ALP activity of osteoblasts MC3T3-E1; respectively collecting chemical components, components and disease-related targets of ginseng and mulberry through a traditional Chinese medicine component database TCMSP, a GeneCards and an OMIM database, taking an intersection of the components and the disease-related targets, analyzing protein interaction between key targets by applying an STRING database, and carrying out biological function and pathway analysis by utilizing a DAVID database; adopting Cytoscape 3.7.0 to perform composition and network topology structure analysis on a related result; and carrying out molecular docking on potential effective substances and the key targets by using an Autodock software.

Description

technical field [0001] The invention belongs to the technical field of herbal medicine pair research, and in particular relates to research on in vitro pharmacodynamics combined with network pharmacology and molecular docking technology and exploration of the mechanism of ginseng-mulberry medicine on osteoporosis. Background technique [0002] Osteoporosis (OP) is characterized by disordered bone metabolism and microstructural damage, and due to loss of bone mass and strength, it is easy to be complicated by fragility fractures in major parts in the future. The incidence of OP is increasing year by year with the aggravation of population aging. It is now the seventh most common disease in the world. Studies have shown that traditional Chinese medicine and its compound have an overall regulatory effect, regulate hormone levels, improve immunity, promote bone density, improve and repair damaged trabecular bone microstructure, and treat diseases in OP patients. It has high sa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/605A61P19/10G16C20/50G16C20/70C12Q1/02G01N21/31G01N33/573
CPCA61K36/605A61K36/258A61P19/10G16C20/50G16C20/70G01N33/5044G01N21/31G01N33/573G01N2333/916G01N2500/10A61K2300/00
Inventor 张辉李春楠刘玲吴楠杜延佳李晶峰李志成边学峰吕金朋兰梦高旭
Owner CHANGCHUN UNIV OF CHINESE MEDICINE
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