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Bifidobacterium breve capable of relieving diarrhea and application thereof

A technology of Bifidobacterium breve and diarrhea, applied in the field of microorganisms, to achieve the effect of reducing the level of pro-inflammatory factors, increasing the diversity of intestinal flora, and low cost

Active Publication Date: 2021-06-22
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, current experimental results are not strong enough to support an oral cholera vaccine Diarrhea caused by ETEC can be prevented, which may be due to the diversity of O and H antigens of ETEC serotypes

Method used

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  • Bifidobacterium breve capable of relieving diarrhea and application thereof
  • Bifidobacterium breve capable of relieving diarrhea and application thereof
  • Bifidobacterium breve capable of relieving diarrhea and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Example 1: Screening and strain identification of Bifidobacterium breve CCFM1151

[0056] 1. Screening

[0057] Taking healthy human feces samples from Fengqiu area, Henan Province, take 0.5g samples preserved in 30% (v / v) glycerol, add 4.5mL normal saline (containing 0.5g / L cysteine acid) in a 10mL centrifuge tube to obtain 10 -1 Diluent, repeat the above dilution steps to obtain 10 -2 、10 -3 、10 -4 、10 -5 、10 -6 Dilution: Pipette 100 μL of gradient dilutions of different gradients and spread them on MRS solid medium (containing 0.5g / L cysteine), incubate at 37°C for 72 hours to obtain a dilution-coating plate; pick a dilution-coating plate The typical colonies above were streaked on the MRS solid medium (containing 0.5 g / L cysteine), cultured at 37°C for 48 hours, and purified colonies were obtained; the purified colonies were picked and inoculated into MRS liquid medium (containing 0.5 g / L cysteine), cultured at 37°C for 48 hours to obtain strain CCFM1151.

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Embodiment 2～5

[0060] In embodiment 2~5, the preparation method of lactic acid bacteria liquid is as follows:

[0061] Dip the lactic acid bacteria liquid to streak on the MRS solid medium, culture at 37°C for 48 hours to obtain a single colony; pick a single colony and insert it into the MRS liquid medium, and cultivate it at 37°C for 24 hours to obtain an activation solution; Inoculate the inoculum of % (v / v) into the MRS liquid medium, and cultivate it at 37°C for 24 hours to obtain the primary seed liquid; insert the primary seed liquid into the MRS liquid culture according to the inoculation amount of 1% (v / v) culture medium at 37°C for 24 hours to obtain a secondary seed liquid; insert the secondary seed liquid into the MRS liquid medium according to the inoculation amount of 1% (v / v), and cultivate at 37°C for 24 hours to obtain a bacterial liquid; The bacterial solution was centrifuged at 6000g for 15min to collect the precipitate; the precipitate was washed twice with PBS buffer sol...

Embodiment 2

[0063] Example 2: Effects of Bifidobacterium breve CCFM1151 on body weight and feces water content of mice with diarrhea

[0064] Specific steps are as follows:

[0065] Take 48 female pathogen-free (SPF) BALB / c mice aged 3-4 weeks, and raise them under the conditions of room temperature 22-24°C, humidity 40-60%, 12h / 12h day and night, free access to food and water After feeding for 1 week, they were randomly divided into 6 groups with 8 rats in each group. The 6 groups were respectively: control group, model group, ciprofloxacin-administered drug group, Lactobacillus rhamnosus (Lactobacillus rhamnosus) GG LGG group, the CCFM1151 group of oral administration of Bifidobacterium breve (Bifidobacterium breve) CCFM1151 bacterium liquid, the FJSWX38M7 group of oral administration of Bifidobacterium breve (Bifidobacterium breve) FJSWX38M7 bacterial liquid, wherein Bifidobacterium breve FJSWX38M7 is screened according to the same method as in Example 1 Methods The feces samples of i...

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Abstract

The invention discloses bifidobacterium breve capable of relieving diarrhea and an application of the bifidobacterium breve and belongs to the technical field of microorganisms. According to the invention, a strain of bifidobacterium breve CCFM1151 is screened, and the bifidobacterium breve CCFM1151 has the effect of relieving diarrhea caused by ETEC, which is specifically reflected as follows: (1) the weight loss of diarrhea mice is obviously reduced; (2) the water content of excrement of diarrhea mice is obviously reduced; (3) the proinflammatory factor level in serum of diarrhea mice is obviously reduced; (4) the content of short-chain fatty acid in excrement of diarrhea mice is obviously increased; and (5) the bifidobacterium breve CCFM1151 obviously improves the intestinal flora diversity of diarrhea mice, so that the bifidobacterium breve CCFM1151 has a huge application prospect in preparation of products (such as food or medicines) for preventing and / or treating diarrhea caused by ETEC.

Description

technical field [0001] The invention relates to a strain of bifidobacterium breve capable of alleviating diarrhea and application thereof, belonging to the technical field of microorganisms. Background technique [0002] According to epidemiological analysis, diarrhea is one of the most common digestive system diseases, and bacterial diarrhea occupies the main position. The most common bacillary dysentery pathogens include Escherichia coli, Salmonella, Shigella and Staphylococcus aureus. Enterotoxigenic Escherichia coli (ETEC) is a major cause of traveler's diarrhea as well as diarrhea and stunting specific to children in developing countries. ETEC produces heat-labile (LT) and heat-stable (ST) enterotoxins, which are structurally and functionally similar to anticholera toxins, stimulate cAMP synthesis in cells, and cause diarrhea in humans and other animals by colonizing the small intestine. [0003] For the treatment of diarrhea, the use of antibiotics is an effective mea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/745A23C9/123A23C13/16A23C15/06A23C17/02A23C19/032A23C19/06A23C21/02A23L13/40A23L19/00A23L11/50A23L11/45A23L11/65A23L33/135A61P1/12C12R1/01
CPCC12N1/20A61K35/745A61P1/12A23C9/1234A23C11/106A23C13/16A23C15/065A23C17/02A23C19/0323A23C19/062A23C20/025A23C21/026A23L13/45A23L19/00A23L33/135A23V2002/00A23V2400/519A23V2200/32Y02A50/30
Inventor 杨波陈卫何竹筠岳月赵建新张灏
Owner JIANGNAN UNIV
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