Method for detecting glutaraldehyde by using ultra-high performance liquid chromatography-tandem mass spectrometry

A technology of ultra-high performance liquid chromatography and tandem mass spectrometry, which is applied in the field of ultra-high performance liquid chromatography-tandem mass spectrometry to detect glutaraldehyde, to achieve the effect of reducing the detection limit, avoiding matrix interference, and good selectivity

Inactive Publication Date: 2021-06-18
江苏乾元生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is a lack of an ultra-high performance liquid chromatography-tandem mass spectrometry method with high detection sensitivity, simplicity, rapidity, and ease of operation to effectively detect residual glutaraldehyde in drugs.

Method used

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Embodiment Construction

[0017] In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are part of the present invention Examples, not all examples.

[0018] The invention provides a method for detecting glutaraldehyde using ultra-high performance liquid chromatography-tandem mass spectrometry, which is characterized in that it comprises the following steps:

[0019] In step (1), take 0.05-0.10 g of the sample to be tested, accurate to 0.01 mg. Place in a volumetric flask, add acetonitrile-water (3-9): 1 to make up to 20-40ml, vortex and mix for 4-9min, ultrasonically extract for 5-10min, pass through a 0.22μm filter membrane, inject the sample to correct Aldehyde detection;

[0020] In step (2), glutaraldehyde is detected by u...

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Abstract

The invention relates to a method for detecting glutaraldehyde by using ultra-high performance liquid chromatography-tandem mass spectrometry. The method comprises the following steps: (1), putting a sample to be detected into a volumetric flask, adding acetonitrile-water (3-9): 2 to fix the volume to 10-50 ml, carrying out vortex mixing for 7-13 min, carrying out ultrasonic extraction for 7-13 min, passing through a 0.22 [mu]m filter membrane, and injecting the sample to detect glutaraldehyde in the sample; and (2) detecting the glutaraldehyde by adopting an ultra-high performance liquid chromatograph-tandem mass spectrometer (UPLC-MS / MS) as a detection instrument. The detection method has the advantages of good selectivity, high sensitivity, simplicity, rapidness and the like. According to the invention, the UPLC-MS / MS can be used for rapidly and efficiently carrying out limited test on glutaraldehyde in bulk drugs, pharmaceutical preparations and other samples; and the detection sensitivity of glutaraldehyde can be effectively improved, matrix interference is effectively avoided, and the detection limit of glutaraldehyde is reduced.

Description

technical field [0001] The invention relates to a detection method for glutaraldehyde, in particular to a method for detecting glutaraldehyde by using ultra-high performance liquid chromatography-tandem mass spectrometry. Background technique [0002] Glutaraldehyde, molecular formula C5H8O2, colorless transparent oily liquid with pungent smell, soluble in hot water. Strong irritant to eyes, skin and mucous membranes. It can be used as a processing aid in the food industry, a disinfectant, a leather tanning agent, a wood preservative, a drug and a raw material for polymer synthesis, etc. For people who have been exposed to glutaraldehyde for a long time, glutaraldehyde can cause local skin and mucous membrane irritation, and it has been reported to cause allergic contact dermatitis, asthma, epistaxis, rhinitis, etc. Therefore, thick gloves should be worn when contacting glutaraldehyde solution Rubber gloves and goggles to prevent splashing liquid in eyes. In order to redu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/06
CPCG01N30/06G01N30/88
Inventor 刘长生肖石基
Owner 江苏乾元生物科技有限公司
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