A kind of ultra-low pressure membrane separation system and separation method

Abstract

The invention discloses an ultra-low pressure membrane separation system, comprising an ultra-low pressure generating device, a membrane scale control module, a flow rate control device, and a pressure detection device; the ultra-low pressure generating device is configured to be used to provide The required ultra-low pressure, the ultra-low pressure is between 0.01KPa and 40KPa; the membrane scale control module is configured to keep the membrane scale layer in a single layer or loose state; the flow rate control device is configured In order to be able to control the flow rate of the sample to be filtered, the pressure detection device is configured to be able to detect the magnitude of the pressure. The invention also discloses an ultra-low pressure membrane separation method. The membrane separation system of the invention can accurately ensure the dynamic stability of the membrane separation system and control it at the ultra-low pressure separation level, and the system can be used to separate biological samples. The separation efficiency is improved, the separation time is shortened, and the cell state is not damaged or changed during the separation process.

Description

technical field [0001] The invention specifically relates to an ultra-low pressure membrane separation system and a separation method. Background technique [0002] From the perspective of clinical sample separation methodology, precise separation methods mainly include: density gradient centrifugation, microfluidic chip separation, flow cytometry, and magnetic bead sorting. Considering the essence of separation, there are two main types: (1) sieving by physical properties (such as density, hardness, size, shape, etc.), and (2) specific adsorption separation by biochemical properties. [0003] As a comprehensive separation method, membrane separation can separate target objects by physical or biochemical properties. The common membrane separation or membrane filtration field is divided into methods such as reverse osmosis filtration, nanofiltration, ultrafiltration and microfiltration. In the biological field, there are membrane separation methods such as separation of nucl...

AI Technical Summary

Problems solved by technology

However, microporous membranes such as chemical membranes or nuclear pore membranes are limited by the support strength and pore size precision. Increasing the membrane pore size has to reduce the porosity of the membrane, so the actual macroscopic filtration efficiency of the fluid passing through the membrane may be reduced instead. If these adsorption effects and non-specific interception are increased, the ultimate benefit of separating clinical or biological samples by ultra-low pressure membranes may not be increased.

Therefore, the existing membrane separation system is unable to perform high-precision, effective and non-destructive separation of cell subpopulations with certain size or stiffness characteristics.

[0006] In addition, the existing membrane separation technology generally requires a large membrane pressure to ensure the filtration flux. Conventional microfiltration or ultrafiltration is generally carried out under a pressure above MPa to ensure a certain filtration rate and a stable filtration rate of the sample. membrane scale

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