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Micropterus salmoides breeding individual marking method

A technology of largemouth bass and marking method, which is applied in fish farming, application, animal husbandry, etc., can solve the problems such as the survival rate and detection rate need to be improved, the marking detection efficiency is reduced, and the fish body is damaged, and the realization of Controllability of mating, improvement of retention rate and detection rate, effect of infection prevention

Active Publication Date: 2021-06-08
FRESHWATER FISHERIES RES INST OF SHANDONG PROVINCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, the first is to use external markers, however, the external markers cause great damage to the fish body and are easy to fall off
The second is to inject electronic markers. However, the previous patents that used fish injection markers all used the method of intraperitoneal injection. After growing up, the muscle layer becomes thicker and viscera increases, and the detection efficiency of markers decreases accordingly. For example, "a method for marking individual grass carp selective breeding" (application number: CN201010582254.0, publication number: CN102077794A, has been submitted by Shen Yubang, Shanghai Ocean University, etc. Ineffective) adopts intraperitoneal injection, the survival rate after marking is 98.6%, and the detection rate is 98% after one year, "a kind of tilapia individual marking method" (Application No. : CN200810018667.9, authorization announcement number: CN101238800B, expired), the survival rate after marking is 99%, and the detection rate is 97% after one year, and the survival rate and detection rate still need to be improved
At the same time, the existing intraperitoneal injection method needs to take the fish out of the water and scan the abdomen to read the electronic tag

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] (1) Selection and holding of fish

[0031] Select largemouth bass weighing more than 30g for marking; before marking, largemouth bass must be kept for one day and cannot eat.

[0032] (2) Pretreatment

[0033] Select the RFID (radio frequency identification) electronic tag as the tag. The diameter of the RFID electronic tag is about 1.25mm and the length is 7mm. Use alcohol with a mass concentration of 70% to disinfect the RFID electronic tag, syringe, and hands, and prepare an inner flat cushion Medical white porcelain tray, the medical white porcelain tray is filled with saline with a mass concentration of 0.3%, and the saline is submerged in the cushion.

[0034] (3) Fish body anesthesia

[0035] Anesthetize the largemouth bass before marking. The anesthetic used is eugenol with a concentration of 20mg / L. During anesthesia, the largemouth bass is immersed in the anesthetic; when the fish body begins to lose balance in the water, it floats or lies on its side in the...

Embodiment 2

[0044] (1) Selection and holding of fish

[0045] Select largemouth bass weighing more than 30g for marking; before marking, largemouth bass must be kept for one day and cannot eat.

[0046] (2) Pretreatment

[0047]Select the RFID (passive integrated transponder) electronic tag as the tag. The diameter of the RFID electronic tag is about 2.12mm and the length is 12mm. Use iodophor to disinfect the RFID electronic tag, syringe, and hands, and prepare a medical white porcelain tray with a soft cushion inside. The medical white porcelain tray is filled with saline with a mass concentration of 0.5%, and the saline is submerged in the cushion.

[0048] (3) Fish body anesthesia

[0049] Anesthetize the largemouth bass before marking. The anesthetic used is eugenol with a concentration of 30 mg / L. During anesthesia, the largemouth bass is immersed in the anesthetic; when the fish body begins to lose balance in the water, it floats or lies on its side in the water , the gill cover...

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Abstract

The invention discloses a micropterus salmoides breeding individual marking method, and belongs to the technical field of fish breeding. The micropterus salmoides breeding individual marking method comprises the following steps of fish selection and temporary rearing, pretreatment, fish body anesthesia, fish body marking, mark detection, anesthesia recovery and medicated bath. Compared with the prior art, the micropterus salmoides breeding individual marking method has the beneficial effects that damage to marked fish bodies can be reduced to the maximum extent, the retention rate of the marks can be increased, meanwhile, it is guaranteed that the positions of the marks are not changed, the fishes are still easy to detect through a detector even if the fishes grow up, and the influence on normal activities of the fish bodies in the breeding process is extremely small; and in the breeding of the micropterus salmoides, the conditions of group source, pedigree, production parameters and the like of each fish are tracked by marking, and different polyculture individuals and families are identified during detection related to economic characters, so that the accuracy of genetic parameter estimation is improved, an identity marker is provided for a breeding parent of the fish, the mating controllability of the micropterus salmoides is realized, and the inbreeding decline is avoided.

Description

technical field [0001] The invention relates to the technical field of fish breeding, in particular to a method for marking individuals of largemouth bass breeding. Background technique [0002] Largemouth bass is native to the Mississippi River system in California, USA. It was introduced to mainland my country in 1983. Because of its delicious meat, rapid growth, and wide temperature adaptability, it has been continuously expanded in the national breeding scale and production in recent years. It has become an important species in my country. One of the freshwater aquaculture species, the industry is developing steadily. Since my country is not the origin of largemouth bass, most of the broodstock used in the seed production process are imported offspring. After generations of breeding and inbreeding, the germplasm degradation of the offspring seedlings is relatively serious, and the hatching rate and emergence rate have already appeared. The degradation of traits such as re...

Claims

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Application Information

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IPC IPC(8): A01K61/95
CPCA01K61/95Y02A40/81
Inventor 李娴朱永安朱树人于振海安丽刘峰孟庆磊董学飒
Owner FRESHWATER FISHERIES RES INST OF SHANDONG PROVINCE
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