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Method for simultaneously determining contents of chlorogenic acid, caffeic acid, ferulic acid and chicoric acid in chicory

A technology of chlorogenic acid and caffeic acid, which is applied to the content of ferulic acid and chicory acid, caffeic acid, and the field of simultaneous determination of chlorogenic acid in chicory, can solve the problems of long detection time, increased instrument cost, and limited measurement range, and achieves The method is simple, the cost of the instrument is reduced, and the peak area is suitable for the effect

Pending Publication Date: 2021-06-04
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above determination methods are for the simultaneous determination of multiple components, the detection time is relatively long (50, 50, 34, 55 and 40 min respectively), and the elution method is gradient elution, which requires the determination instrument to be equipped with a gradient elution device, which increases the cost of the instrument , the measurement range is limited by the equipment

Method used

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  • Method for simultaneously determining contents of chlorogenic acid, caffeic acid, ferulic acid and chicoric acid in chicory
  • Method for simultaneously determining contents of chlorogenic acid, caffeic acid, ferulic acid and chicoric acid in chicory
  • Method for simultaneously determining contents of chlorogenic acid, caffeic acid, ferulic acid and chicoric acid in chicory

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Step 1: Preparation of the solution solution

[0039] The chlorogenic acid, coffee acid, ferulic acid and chicninic acid, precision weighted, placed in a brown volumetric flask, adding methanol-0.5% phosphoric acid (3: 1, v / v) solution, and is made of 0.125 mg / ML chlorogenic acid, caffeic acid, ferulic acid and chicninic acid reservoir, as the stock solution, the other different quality concentrations of reaction solution is diluted by the stock solution;

[0040] Step 2: Preparation of the test solution

[0041]When the chicory powder was prepared, it was precisely 40 megadantica powder 5g, and 250 ml of methanol-0.5% phosphate (3: 1, v / v) solution was added, and the ultrasonic ultrasonic treatment was 200W, the vacuum was 50 Pa, and the vacuum was 50 Pa. The reaction kettle temperature is 20 ° C, the ultrasonic frequency is 10 kHz, the treatment time is 75min, and the treatment time is filtered, the filtrate is filtered, the filtrate is a 0.22 μm microporous filter,...

Embodiment 2

[0045] Step 1: Preparation of the solution solution

[0046] The chlorogenic acid, coffee acid, ferulic acid and chicnichic acid, precision weighted, placed in a brown volumetric flask, adding methanol-0.5% phosphoric acid (4: 1, v / v) solution to prepare 0.15 mg / ML chlorogenic acid, caffeic acid, ferulic acid and chicninic acid reservoir, as the stock solution, the other different quality concentrations of reaction solution is diluted by the stock solution;

[0047] Step 2: Preparation of the test solution

[0048] When the chicory powder is 50.5 g of a chicory powder, 7.5 g of the chicory powder is precisely weighted, 400 ml of methanol-0.5% phosphate (4: 1, v / v) solution, vacuum ultrasonic treatment, the ultrasonic treatment power is 300W, the vacuum is 80Pa The reaction kettle temperature is 18 ° C, the ultrasonic frequency is 15 kHz, the time is 70 min, and the treatment is filtered, the filtrate is filtered, the filtrate is 0.22 μm microporous filter, that is, the test ...

Embodiment 3

[0052] Step 1: Preparation of the solution solution

[0053] The chlorogenic acid, coffee acid, ferulic acid and chicninic acid, precision weighted, placed in a brown volumetric flask, adding methanol-0.5% phosphate (4: 1, v / v) solution, 0.25 mg / ML chlorogenic acid, caffeic acid, ferulic acid and chicninic acid reservoir, as the stock solution, the other different quality concentrations of reaction solution is diluted by the stock solution;

[0054] Step 2: Preparation of the test solution

[0055] The chicory powder was precisely referred to when the chicory powder was sieved, and the chicory powder was accurately weighed, 500 ml of methanol-0.5% phosphate (4: 1, v / v) solution, vacuum ultrasonic treatment, the ultrasonic treatment power is 400W, the vacuum is 100 Pa, The reaction kettle temperature was 15 ° C, the ultrasonic frequency was 20 kHz, and the time was 60 min, and the treatment was filtered, the filtrate was filtered, and the test solution was obtained by a 0.22 ...

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Abstract

The invention discloses a method for simultaneously determining the contents of chlorogenic acid, caffeic acid, ferulic acid and chicoric acid in chicory, and belongs to the technical field of content determination of active ingredients of traditional Chinese medicines. The method comprises the following steps: preparation of a reference substance solution, preparation of a test solution and quantitative analysis; an HPLC method is adopted, the content of chlorogenic acid, caffeic acid, ferulic acid and chicoric acid in the chicory is measured at the same time in an isocratic elution mode, wave crest separation is good, the peak area size is appropriate, and peak appearance is completed within about 20 min after one injection of a sample; the method is simple, convenient, rapid, accurate, low in cost, wide in application range and easy to popularize and master.

Description

Technical field [0001] The present invention relates to the field of traditional Chinese medicine active ingredient content, and more particularly to a method of simultaneously determining chlorosic acid, caffeic acid, ferulic acid and chrysome acid content in chicory. Background technique [0002] Chicory (CICHORUM INTYBUS L.) is a born herbaceous plant. In the "China Pharmacopoeia" (2020 edition), chicory as the Uighur learning medicine, mainly using its dry ground or root, with clear liver gallbladder, joining digestion, diuretic swelling, used for wet heat jaundice, stomach pain And the urine is less. There are many uses, and the leaves can be used as vegetables and feed, and the roots can be used to produce daisy and coffee alternatives. Chicory contains a variety of phenolic compounds such as chlorogenic acid, caffeic acid, ferulic acid, and chicnia, with free radicals, anti-lipid oxidation, protective liver, antibacterial, antiviral, enhanced immune function and Anti-infla...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/74
CPCG01N30/06G01N30/74
Inventor 尚红梅田雨张南翼刘梦雪李欣雨鲍晨光
Owner JILIN AGRICULTURAL UNIV
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