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Quantitative determination method of chlorogenic acid and three kinds of isochlorogenic acid in lonicera flower medicinal material and preparation thereof

A technology for the quantitative determination of isochlorogenic acid, applied in the direction of measuring devices, material separation, and analytical materials, etc., can solve problems such as the inability to correctly evaluate the overall quality of medicinal materials and preparations, and achieve multi-index control of preparation quality, good separation, and high resolution Improved effect

Active Publication Date: 2013-05-08
承德燕峰药业有限责任公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Both chlorogenic acid and isochlorogenic acid are active ingredients of honeysuckle, and their spectral scans are basically the same (see figure 1 , figure 2 , image 3 , Figure 4 ), and the sum of the contents of isochlorogenic acids A, B, and C is about 70% to 110% of chlorogenic acid, so only measuring chlorogenic acid cannot correctly evaluate the overall quality of medicinal materials and preparations

Method used

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  • Quantitative determination method of chlorogenic acid and three kinds of isochlorogenic acid in lonicera flower medicinal material and preparation thereof
  • Quantitative determination method of chlorogenic acid and three kinds of isochlorogenic acid in lonicera flower medicinal material and preparation thereof
  • Quantitative determination method of chlorogenic acid and three kinds of isochlorogenic acid in lonicera flower medicinal material and preparation thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] 1. Chromatographic conditions and system suitability test: Acetonitrile-methanol-0.1% phosphoric acid with a volume ratio of 15:6.5:78.5 is used as the mobile phase; column temperature: 40°C; detection wavelength is 326nm; the number of theoretical plates is calculated according to the peak of chlorogenic acid Should not be less than 2000;

[0067] 2. Preparation of reference substance solution Take appropriate amount of reference substances of chlorogenic acid, isochlorogenic acid A, isochlorogenic acid B, and isochlorogenic acid C respectively, weigh them accurately, put them in a brown measuring bottle, and add methanol to make each 1ml A solution containing 40 μg of chlorogenic acid, 20 μg of isochlorogenic acid A, 10 μg of isochlorogenic acid B, and 5 μg of isochlorogenic acid C was used as a reference solution;

[0068] 3. Preparation of the test solution Take 0.1g of honeysuckle medicinal material fine powder, accurately weigh it, put it in a stoppered Erlenmeyer...

Embodiment 2

[0071] 1. Chromatographic conditions and system suitability test: Acetonitrile-methanol-0.1% phosphoric acid with a volume ratio of 14.5:6.3:79.2 is used as the mobile phase; column temperature: 40°C; detection wavelength is 326nm; the number of theoretical plates is based on the peak of chlorogenic acid A The calculation should not be less than 2000;

[0072] 2. Preparation of reference substance solution Take appropriate amount of reference substances of chlorogenic acid, isochlorogenic acid A, isochlorogenic acid B, and isochlorogenic acid C respectively, weigh them accurately, put them in a brown measuring bottle, and add methanol to make each 1ml Containing 40 μg of chlorogenic acid, a solution of 12 μg each of isochlorogenic acid A, isochlorogenic acid B, and isochlorogenic acid C is used as a reference solution;

[0073] 3. Preparation of the test solution Take 0.5g of sucrose-free infant pharynx flat granules, accurately weigh it, put it in a stoppered Erlenmeyer flask...

Embodiment 3

[0076] 1. Chromatographic conditions and system suitability test: Acetonitrile-methanol-0.1% phosphoric acid with a volume ratio of 14:6:80 is used as the mobile phase; column temperature: 40°C; detection wavelength is 326nm; the number of theoretical plates is based on the peak of chlorogenic acid A The calculation should not be less than 2000;

[0077]2. Preparation of reference substance solution Take appropriate amount of reference substances of chlorogenic acid, isochlorogenic acid A, isochlorogenic acid B, and isochlorogenic acid C respectively, weigh them accurately, put them in a brown measuring bottle, add methanol to make each 1ml Containing 40 μg of chlorogenic acid, a solution of 12 μg each of isochlorogenic acid A, isochlorogenic acid B, and isochlorogenic acid C is used as a reference solution;

[0078] 3. Preparation of the test solution: 1.0g of sucrose-containing infant pharynx flat granules, accurately weighed, placed in a stoppered Erlenmeyer flask, accurate...

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Abstract

The invention relates to a quantitative determination method of chlorogenic acid and three kinds of isochlorogenic acid in lonicera flower medicinal material and preparation thereof, which is characterized in that: ordinary isocratic elution is adopted at the first time, acetonitrile-methanol-0.1% phosphoric acid with the volume ratio of (15 to 14): (6.5 to 6): (78.5 to 80) is adopted as a flow phase, and the content of the chlorogenic acid and three kinds of isochlorogenic acid in the lonicera flower medicinal material and preparation thereof is simultaneously determined at 326 minus or plus2nm. The method is simple, convenient and rapid to operate and is easy to popularize and master; separation of each peak of a quantitative chromatogram map of each component on three different chromatographic columns is good, the basic line is stable, and the peak appearance is completed within 20 to 30 minutes. Compared with the gradient elution, the cost of instruments is greatly reduced, the popularization rate of the method is improved, and the determination time is reduced. Due to the adoption of the quantitative determination method, a determination method and reference data can be supplied for evaluating the quality of the lonicera flower medicinal material and the preparation thereof through multiple indexes.

Description

technical field [0001] The invention belongs to the field of quality control of traditional Chinese medicines, and relates to a quality detection method of honeysuckle medicinal materials and preparations thereof, in particular to a quantitative determination method of chlorogenic acid and three kinds of isochlorogenic acids in honeysuckle medicinal materials and preparations thereof. Background technique [0002] Honeysuckle is the dry flower bud or the first flower of Lonicera japonica Thunb. It is a commonly used traditional Chinese medicine, which has the effect of clearing away heat and detoxification, and evacuating wind-heat. For carbuncle swollen furuncle, sore throat, erysipelas, pyretic toxin bloody dysentery, wind-heat cold, febrile disease heating. The preparation refers to the sucrose-containing and sucrose-free Xiaoeryanbian granules, which are used for children and are listed in the Chinese Pharmacopoeia 2010 Edition. It is used for children with sore throat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/34
Inventor 韩桂茹安丽娜申玉龙刘勇
Owner 承德燕峰药业有限责任公司
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