Kit of double RPA detection of aphthovirus and vesicular stomatitis virus

Abstract

The invention discloses a kit of double RPA detection of aphthovirus and vesicular stomatitis virus. The kit includes an aphthovirus RPA detection primer set consisting of nucleotide sequences shown as SEQ. ID. NO. 1 and SEQ. ID. NO. 6 and a vesicular stomatitis virus RPA detection primer set consisting of nucleotide sequences shown as SEQ. ID. NO. 8 and SEQ. ID. NO. 11. Based on a recombinase polymerase amplification method, the kit takes the cDNA formed by RNA reverse transcription as a template and performs amplification reaction under optimized reaction temperature and time, and detection results can be obtained through nucleic acid gel electrophoresis. The kit can be used to simultaneous detect aphthovirus and vesicular stomatitis virus, is simple in operation and has high sensibility and specificity, therefore rapid detection kit can be provided for on-site screening of pathogens.

Description

technical field [0001] The invention relates to the field of viral nucleic acid detection, in particular to a double RPA detection primer set, kit and detection method for foot-and-mouth disease virus and vesicular stomatitis virus. Background technique [0002] Foot-and-mouth disease virus (FMDV) is a member of the genus FMDV in the Picornaviridae family and mainly infects cattle, sheep, pigs and other animals. Vesicular stomatitis virus (VSV) is a member of the Rhabdoviridae Vesicularvirus genus and is an enveloped, non-segmented, negative-sense RNA virus. Both vesicular stomatitis and foot-and-mouth disease can cause vesicular lesions on the oral mucosa, nipple skin, and hoof crown skin of animals. The clinical symptoms are similar and it is difficult to distinguish them. Because it is difficult to distinguish between foot-and-mouth disease and vesicular stomatitis clinically, and these two viruses have brought great harm to the breeding industry and have a serious impac...

AI Technical Summary

Problems solved by technology

However, the detection of FMDV and VSV at the same time mainly involves gene chip and PCR technology, and there is no report involving RPA technology

Compared with PCR, RPA has more stringent requirements on primer design, which cannot be realized completely according to software design. Screening effective specific amplification primers and reaction conditions is a difficult problem in realizing multiple RPA detection.

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