Foot and mouth disease virus and vesicular stomatitis virus dual rpa detection kit

Abstract

The invention discloses a double RPA detection kit for foot-and-mouth disease virus and vesicular stomatitis virus, which comprises foot-and-mouth disease virus RPA detection primers composed of nucleotide sequences shown in SEQ.ID.NO.1 and SEQ.ID.NO.6 group, and a primer group for vesicular stomatitis virus RPA detection consisting of the nucleotide sequences shown in SEQ.ID.NO.8 and SEQ.ID.NO.11. The kit is based on the recombinase polymerase amplification method, uses the cDNA formed by reverse transcription of RNA as a template, performs amplification reaction under optimized reaction temperature and time, and uses nucleic acid gel electrophoresis to obtain detection results. The invention can be used to detect foot-and-mouth disease virus and vesicular stomatitis virus simultaneously, has simple operation, high sensitivity and specificity, and provides a rapid detection reagent for on-site screening of pathogens.

Description

technical field [0001] The invention relates to the field of viral nucleic acid detection, in particular to a dual RPA detection primer set, kit and detection method for foot-and-mouth disease virus and vesicular stomatitis virus. Background technique [0002] Foot-and-mouth disease virus (FMDV) is a member of the genus Foot-and-mouth disease virus of the Picornaviridae family, mainly infecting cattle, sheep, pigs and other animals. Vesicular stomatitis virus (VSV) is a member of the Rhabdoviridae family, Vesicular stomatitis virus (VSV), and is an enveloped, non-segmented, negative-sense RNA virus. Both vesicular stomatitis and foot-and-mouth disease can cause vesicular lesions on the oral mucosa, nipple skin and hoof crown skin of animals, with similar clinical symptoms and difficult to distinguish. Because it is difficult to distinguish foot-and-mouth disease and vesicular stomatitis in clinic, and these two viruses have brought great harm to the breeding industry and ha...

AI Technical Summary

Problems solved by technology

However, the detection of FMDV and VSV at the same time mainly involves gene chip and PCR technology, and there is no report involving RPA technology

Compared with PCR, RPA has more stringent requirements on primer design, which cannot be realized completely according to software design. Screening effective specific amplification primers and reaction conditions is a difficult problem in realizing multiple RPA detection.

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