Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A method for evaluating the resistance of masson pine provenance to pine xylophilus disease

A technology for pine wood nematode and pine wood nematode, which is applied in the field of evaluating the resistance of masson pine provenance to pine wood nematode, and can solve the problems of difficulty in identifying the source of masson pine provenance and frequent seedling transportation

Active Publication Date: 2022-06-03
JIANGSU ACAD OF FORESTRY
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the source of the provenance of the disease-resistant Pinus massoniana is known, it is difficult to distinguish the source of the provenance of Pinus massoniana due to the frequent transfer of seedlings

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for evaluating the resistance of masson pine provenance to pine xylophilus disease
  • A method for evaluating the resistance of masson pine provenance to pine xylophilus disease
  • A method for evaluating the resistance of masson pine provenance to pine xylophilus disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment example 1

[0029] A method for evaluating the resistance of masson pine provenance to pine wood nematode, comprising the following steps:

[0030] (1) Prepare a PDA medium, inoculate Botrytis cinerea on the medium, culture at 25°C for 5-7 days, inoculate the pine wood nematodes after the mycelium of Botrytis cinerea is covered on the plate, and culture at 25°C for 5-7 days , after the pine wood nematodes multiply and eat the mycelium of Botrytis cinerea, wash out the pine wood nematodes with sterile water, and centrifuge and concentrate to prepare a nematode suspension of about 5000 / mL;

[0031] (2) Use a blade to cut a downward wound to the xylem at a convenient height on the trunk of Masson pine, insert cotton into the wound until the wound is filled, wrap the cotton with tape at the bottom of the wound and stick it around the trunk to form a funnel shape, and then drop the desired nematode suspension on cotton. A masson pine seedling was inoculated with 1 mL of pine wood nematode sus...

Embodiment example 2

[0034] A method for evaluating the resistance of masson pine provenance to pine wood nematode, comprising the following steps:

[0035] (1) Prepare a PDA medium, inoculate Botrytis cinerea on the medium, culture at 25°C for 5-7 days, inoculate the pine wood nematodes after the mycelium of Botrytis cinerea is covered on the plate, and culture at 25°C for 5-7 days , after the pine wood nematodes multiply and eat the mycelium of Botrytis cinerea, wash out the pine wood nematodes with sterile water, and centrifuge and concentrate to prepare a nematode suspension of about 5000 / mL;

[0036] (2) Use a blade to cut a downward wound to the xylem at a convenient height on the trunk of Masson pine, insert cotton into the wound to fill the wound, wrap the cotton with tape at the bottom of the wound and stick it around the trunk to form a funnel shape, and then drop the desired nematode suspension on cotton. A masson pine seedling was inoculated with 1 mL of pine wood nematode suspension,...

Embodiment example 3

[0043] A method for evaluating the resistance of masson pine provenance to pine wood nematode, comprising the following steps:

[0044] (1) Prepare a PDA medium, inoculate Botrytis cinerea on the medium, culture at 25°C for 5-7 days, inoculate the pine wood nematodes after the mycelium of Botrytis cinerea is covered on the plate, and culture at 25°C for 5-7 days , after the pine wood nematodes multiply and eat the mycelium of Botrytis cinerea, wash out the pine wood nematodes with sterile water, and centrifuge and concentrate to prepare a nematode suspension of about 5000 / mL;

[0045] (2) Use a blade to cut a downward wound to the xylem at a convenient height on the trunk of Masson pine, insert cotton into the wound until the wound is filled, wrap the cotton with tape at the bottom of the wound and stick it around the trunk to form a funnel shape, and then drop the desired nematode suspension on cotton. A masson pine seedling was inoculated with 1 mL of pine wood nematode sus...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for evaluating the resistance of masson pine provenance to pine xylophilus disease. Using disease-resistant Pine massoniana GD5 and common Pine massoniana SX1 as materials, through biological observation and inoculation of B. xylophilus infection-induced observation of symptoms, changes in physiological and biochemical substance content, changes in differential protein expression, and differential gene expression, etc., to screen out The biochemical, protein, gene and other indicators related to resistance can be used to evaluate the resistance characteristics of Pine massoniana provenance to B. xylophilus.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for evaluating the resistance of masson pine provenance to pine wood nematode. Background technique [0002] Pine wood nematode disease (Pine Wood Nematode Disease) is one of the major biological disasters that occurs on pine species (Pinus spp.), which can cause massive death of pine trees, seriously damage pine forest resources, natural landscape and ecological environment, and cause serious damage to forestry economy. Heavy losses are important worldwide quarantine objects, and so far there is no economically feasible physical, chemical or biological control method to effectively control them. The selection and breeding of forest tree resistance is an important means to prevent and control the occurrence of forest diseases and insect pests, and it is also a long-term countermeasure. Under the current situation of pine wood nematode disease prevention and control in my co...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/00
CPCA01G17/005A01G7/00A01G7/06C12Q1/6895G01N33/6839C12Q2600/158Y02P60/40
Inventor 郑华英解春霞徐丽丽刘云鹏高悦
Owner JIANGSU ACAD OF FORESTRY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com