Novel multifunctional enzyme gene HG32 and application

Abstract

The invention discloses a novel multifunctional enzyme gene HG32 and application, the enzyme is derived from Chinese rhizomyidae enteric microorganisms, can be compatible with an eukaryotic cell expression system, expresses cellulase with high biological activity, and can be applied to the fields of livestock and poultry feed enzyme preparations, transgenic animal preparation, food and medicine industries and the like; the invention solves the problems that plant cellulose is difficult to degrade, the feed nutrition utilization rate is low and the like are solved, and pollution of nitrogen, organic matter and the like in animal excrement. According to the invention, the application comprises the following steps: sequencing a bamboo rat intestinal microbe macro transcriptome, and splicing the sequences; performing homology comparison with the existing glycoside hydrolase family on NCBI, and screening high-expression suspected cellulase genes with homology higher than 50%; predicting the signal peptide, and replacing the original signal peptide with the pig parotid gland secreted protein signal peptide; optimizing according to pig codon preference, and cloning to an eukaryotic expression vector; transfecting into pig kidney epithelial cells through lipidosome for expression; collecting a cell culture supernatant; and carrying out enzyme activity analysis and comparison.

Description

technical field [0001] The invention relates to the field of genetic engineering, in particular to a novel multifunctional enzyme gene HG32 and its application. Background technique [0002] Cellulose is an abundant green carbon source in nature and the main component of plant cell walls. An appropriate amount of cellulose is beneficial to animal intestinal peristalsis and promotes feces excretion. However, cellulose is also the main component of plant cell walls. Most livestock and poultry are monogastric animals such as pigs and chickens. Their stomachs and small intestines lack cellulase, which makes it difficult to destroy plant cell walls and hinder the full absorption of intracellular nutrients. These Undigested nutrients enter the natural environment with excrement, causing environmental pollution. Naturally, cellulose often exists in a supramolecular aggregate structure and is difficult to be degraded. The way to solve this problem is to find excellent cellulose de...

AI Technical Summary

Problems solved by technology

Thus, a cellulase gene HG32 was obtained, which can be used in livestock and poultry feed enzyme preparations, transgenic animal preparation, food and pharmaceutical industries, etc., to solve the problems of cellulose refractory absorption and excrement polluting the environment

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