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Content detection method of 10 active ingredients in Dendrobium

A detection method and a technology of active ingredients, which are applied in the field of content detection of 10 active ingredients in Dendrobium, can solve problems such as the species, place of origin, and age of Dendrobium, and achieve the effect of ensuring clinical effectiveness, good reproducibility, and rapid method

Active Publication Date: 2022-07-01
INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The species, place of origin, and age of Dendrobium sold in the market are unknown, and its quality detection method is very important for selecting high-quality Dendrobium, but the quality detection method of Dendrobium has not been reported yet

Method used

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  • Content detection method of 10 active ingredients in Dendrobium
  • Content detection method of 10 active ingredients in Dendrobium
  • Content detection method of 10 active ingredients in Dendrobium

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Experimental program
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preparation example Construction

[0049]

[0050] The preparation of the test solution of the present invention is to dissolve the alcohol extract of Dendrobium officinale in a solvent, and then filter it with a microporous membrane to obtain the test solution. The solvent can be selected from one or more of methanol, ethanol, acetonitrile, methanol aqueous solution, ethanol aqueous solution or acetonitrile aqueous solution. Preferably, the volume percentage of methanol in the methanol aqueous solution is 30-95%; the volume percentage of ethanol in the ethanol aqueous solution is 30-95%, and the volume percentage of acetonitrile in the acetonitrile aqueous solution is 30-95%. Preferably, the solvent is methanol. The concentration of the test solution can be 1.0-3.0 mg / mL, preferably 1.5-2.0 mg / mL, more preferably 1.8-2.0 mg / mL. According to a preferred embodiment of the present invention, the concentration of the test solution is 2.0 mg / mL. The concentration of the test solution refers to the ratio of the ...

Embodiment 1

[0107] The quality detection method of embodiment 1 tassel dendrobium

[0108] Preparation of reference solution: Precisely weigh 10 kinds of reference substances, add methanol to dissolve, so that the components in the mixed reference solution are: vanillic acid, syringic acid, vitexin, 2,4,7-trihydroxy- 9,10-dihydrophenanthrene, 7,4'-dihydroxyflavone, liquiritigenin, syringaresinol, naringenin, dendrobium, and eranin, respectively, at a concentration of 50ng / mL, to obtain a reference solution.

[0109] Preparation of the test solution: Weigh an appropriate amount of the alcohol extract of Dendrobium tassel, dissolve with methanol to make the concentration 2mg / mL, and filter with a 0.22 μm microporous membrane to obtain the test solution.

[0110] Ultra-high performance liquid chromatography-mass spectrometry detection: inject the above-mentioned reference solution and test solution into an ultra-high performance liquid chromatography-mass spectrometer, respectively, to obtai...

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Abstract

The invention discloses a method for detecting the content of 10 effective components in Dendrobium officinale. The method adopts an ultra-high performance liquid chromatography-mass spectrometry method for detection. It is composed of acetonitrile A and 0.1% formic acid aqueous solution B, and is eluted by gradient elution; the mass spectrometry is detected by MRM mode. The method of the invention is fast, accurate and reproducible.

Description

technical field [0001] The invention relates to a method for detecting the content of 10 effective components in dendrobium. Background technique [0002] Dendrobium (Dendrobium nobile Lindl.) is a plant of the orchid family Dendrobium. It grows on tree trunks in dense forests or on damp rocks in valleys. It is mainly distributed in Guangxi, Guizhou and Yunnan provinces in my country. Efficacy, clinically used for the treatment of Yin injury and fluid loss, dry mouth and polydipsia, retching due to lack of food, deficiency heat after illness, dark eyesight and other symptoms. The species, origin, and age of Dendrobium sold on the market are unknown, and its quality testing method is very important for selecting high-quality Dendrobium. However, the quality testing method of Dendrobium has not yet been reported. For Dendrobium, the existing literature only reported the method of determination of various phenolic components by ultra-high performance liquid chromatography-mass ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/36G01N30/72G01N30/86
CPCG01N30/02G01N30/06G01N30/34G01N30/36G01N30/7266G01N30/8679
Inventor 孙晶范蓓王凤忠王慧卢聪刘佳萌贾宁孙玉凤金诺
Owner INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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