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Immobilization method of microbial agent

A technology of microbial agent and microbial liquid, which is applied in the direction of fixing on/in the organic carrier, which can solve the problems of poor mass transfer, secondary pollution, and toxic microorganisms

Active Publication Date: 2021-05-18
HANGZHOU NAXI ECOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these carriers have not been widely used in actual restoration projects due to problems such as low strength, small adsorption capacity, poor mass transfer, toxic effects on microorganisms, and easy to cause secondary pollution.

Method used

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  • Immobilization method of microbial agent
  • Immobilization method of microbial agent
  • Immobilization method of microbial agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A method for immobilizing microbial bacterial agents,

[0035] Modification process: add the biological carrier into the hydrogen peroxide solution and oxidize it at a temperature of 25°C for 30 minutes, take it out and add it to an alkaline solution and modify it at a temperature of 25°C for 30 minutes, take it out, wash it and dry it to obtain a modified biological carrier; biological The carrier is corn stalk particles with a particle size of 100 mesh; the concentration of the hydrogen peroxide solution is 3wt%, and the amount of the biological carrier is 10wt% of the hydrogen peroxide solution; the alkaline solution is a sodium hydroxide solution containing sodium phosphate, and the hydrogen peroxide The mass fraction of sodium is 3wt%, the mass fraction of sodium phosphate is 0.5wt%, and the added amount of the biological carrier after oxidation treatment is 10wt% of the sodium hydroxide solution.

[0036] Immobilization process: mix the microbial bacterial liquid ...

Embodiment 2

[0038] Compared with Example 1, this example differs only in that the modified biological carrier is dipped and modified for 3 hours with an immersion solution containing xylo-oligosaccharides; the content of xylo-oligosaccharides in the immersion solution is 6 wt%.

Embodiment 3

[0040] Compared with Example 1, this example differs only in that before the immobilization process, the modified biological carrier is subjected to an immersion modification treatment with an immersion solution containing 2-bornylidene butanol for 3 hours; The content of xylan is 6 wt%, and the content of 2-bornylidene butanol is 1.2 wt%.

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Abstract

The invention discloses an immobilization method of a microbial agent, and belongs to the field of microbial agent preparation. The immobilization method of the microbial agent comprises the following steps: treating a biological carrier through a modification process to obtain a modified biological carrier; and treating microbial liquid, the modified biological carrier and a functional agent through an immobilization process to obtain the immobilized microbial agent, wherein the modification process comprises oxidation treatment. The microbial agent is good in activity, high in ammonia nitrogen removal rate, high in immobilization efficiency and good in preservation stability, the bacterial activity loss rate of the microbial agent reaches 32% or below after the microbial agent is preserved for 3 months, the thermal stability is improved, and the bacterial activity loss rate is 60% or below after the microbial agent is preserved at the temperature of 50 DEG C for 1 h.

Description

technical field [0001] The invention belongs to the field of preparation of microbial bacterial agents, and in particular relates to an immobilization method of microbial bacterial agents. Background technique [0002] In order to improve the shelf life of microbial restoration agents and enhance their adaptability to the environment, immobilization technology is the key. The technology of immobilized microorganisms is mainly the technology of immobilizing strains on the carrier through physical adsorption, embedding method, cross-linking method, etc., among which the selection of appropriate immobilization materials is the key to the immobilization technology. In the physical adsorption immobilization method, the traditional carriers commonly used at present mainly include inorganic materials (such as diatomaceous earth, activated carbon, kaolin, etc.), organic materials (polyvinyl alcohol, polyacrylamide, etc.), natural materials (sodium alginate, shell polysaccharides, s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/02
CPCC12N11/02
Inventor 徐坚麟付源王俊滔
Owner HANGZHOU NAXI ECOLOGICAL TECH CO LTD
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