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Accelerant for improving dry weight of euglena gracilis, euglena gracilis culture medium and application of euglena gracilis culture medium

A technology of accelerator and culture medium, applied in the field of accelerators for improving the dry weight of Euglena, can solve problems such as unsatisfactory dry weight yield of Euglena

Active Publication Date: 2021-05-18
SHENZHEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is a technical problem that the dry weight of euglena is not ideal in the cultivation of euglena at present.

Method used

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  • Accelerant for improving dry weight of euglena gracilis, euglena gracilis culture medium and application of euglena gracilis culture medium
  • Accelerant for improving dry weight of euglena gracilis, euglena gracilis culture medium and application of euglena gracilis culture medium
  • Accelerant for improving dry weight of euglena gracilis, euglena gracilis culture medium and application of euglena gracilis culture medium

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Experimental program
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Effect test

Embodiment 1

[0054] 1. Source of algal species

[0055] The algal species used in the present invention: Euglena. gracilis CCAP 1224 / 5Z, purchased from Culture Collection of Algae and Protozoa.

[0056] 2. Culture medium composition

[0057] See Table 1 for common media formulations for euglena. Microelements a See Table 2 for the formula. The special medium for euglena is the usual medium for euglena plus 15g / L glucose.

[0058] 3. Algae cultivation method

[0059] Pick euglena from the euglena preservation plate and put it into a 150mL conical flask with 50mL of sterilized euglena common medium for activation. After it is cultivated to the logarithmic phase, transfer it to 1L of fresh euglena common medium solution for further expansion. Cultured, placed in a light incubator, cultured at 23±1°C, and the light intensity was 100±5μmol / m 2 the s 1 , continuous light. The whole operation process was carried out in accordance with the standard microbiological experimental method under...

Embodiment 2

[0066] The 2g choline in embodiment 1 is replaced with 2g tetrahydropyrimidine, all the other contents are identical with embodiment 1.

Embodiment 3

[0068] The 2g choline in embodiment 1 is replaced with 1g choline and 1g ectoine, all the other contents are identical with embodiment 1.

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Abstract

The invention provides an accelerant for improving the dry weight of euglena gracilis, an euglena gracilis culture medium and application of the euglena gracilis culture medium, and belongs to the technical field of euglena gracilis culture. The accelerant is selected from one or more of choline, ectoine, vitamin B1 and sulfadiazine. The accelerant is added into a culture medium for euglena gracilis culture to promote dry weight increase of euglena gracilis, biomass efficient output and sustainable culture of euglena gracilis are achieved, research progress of an existing euglena gracilis culture productivity mechanism can be promoted, and the bottleneck problem faced by large-scale euglena gracilis culture is solved.

Description

technical field [0001] The invention relates to the technical field of euglena cultivation, in particular to an accelerator for increasing the dry weight of euglena, a euglena culture medium and applications thereof. Background technique [0002] Euglena gracilis, also known as Euglena gracilis, cells are usually green due to the presence of multiple oval chloroplasts. There are 2 flagella, one protruding from the cell mouth, which is always moving. Fresh water products, spring and summer often reproduce in large numbers in static water bodies with more organic matter, making the water quality green. For example, green eyeworm can be used as a biological indicator of organic pollutants in the environment. Euglena cells have both animal and plant characteristics. Euglena possesses extremely complex metabolic capabilities comparable to multicellular organisms, offering broad opportunities for metabolic engineering to produce value-added biomolecules. After the discovery of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/38C12N1/12C12R1/89
CPCC12N1/38C12N1/12
Inventor 王江新朱嘉怡贺嘉怡秦换刘永启
Owner SHENZHEN UNIV
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