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Nanoreactor as well as preparation method and application thereof

A nanoreactor and nanoparticle technology, applied in biochemical equipment and methods, nanotechnology, nanomedicine, etc., can solve problems such as complex preparation process, increased design difficulty, and tediousness

Active Publication Date: 2021-05-11
湖南省人民医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Designing a multifunctional nanoreactor to simultaneously supply oxygen and remove glutathione and ATP can optimize photodynamic therapy, but the cumbersome material synthesis and complex preparation process increase the design difficulty

Method used

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  • Nanoreactor as well as preparation method and application thereof
  • Nanoreactor as well as preparation method and application thereof
  • Nanoreactor as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] This embodiment provides a method for preparing core-shell nanoparticles, comprising the following steps:

[0060] (1) 40 μL of tannic acid (TA) aqueous solution with a concentration of 40 mg / mL and 10 μL of glucose oxidase (GOx) aqueous solution with a concentration of 10 mg / mL were sequentially added to 5 mL of ultrapure water as the aqueous phase. 100 μL of 20 mg / mL polylactic-co-glycolic acid (PLGA) acetone solution, 40 μL of 10 mg / mL ferric chloride (FeCl 3 ) acetone solution and 20 μL of chlorin e6 (Ce6) dimethyl sulfoxide solution with a concentration of 20 mg / mL were sequentially added to 1 mL of acetone as the organic phase.

[0061] (2) Slowly drop the organic phase into the water phase, ultrasonically stir, and centrifugally wash to obtain core-shell nanoparticles (PTFCG).

Embodiment 2

[0063] The present embodiment provides a kind of preparation method of nanoreactor, comprises the steps:

[0064] (1) 40 μL of tannic acid (TA) aqueous solution with a concentration of 40 mg / mL and 10 μL of glucose oxidase (GOx) aqueous solution with a concentration of 10 mg / mL were sequentially added to 5 mL of ultrapure water as the aqueous phase. 100 μL of 20 mg / mL polylactic-co-glycolic acid (PLGA) acetone solution, 40 μL of 10 mg / mL ferric chloride (FeCl 3 ) acetone solution and 20 μL of chlorin e6 (Ce6) dimethyl sulfoxide solution with a concentration of 20 mg / mL were sequentially added to 1 mL of acetone as the organic phase.

[0065] (2) Slowly drop the organic phase into the water phase, ultrasonically stir, and centrifugally wash to obtain core-shell nanoparticles (PTFCG).

[0066] (3) Take 1 mL of the above-mentioned core-shell nanoparticles, add 50 μL of polypropyleneimine (PAH) with a concentration of 20 mg / mL, and 25 μL of potassium permanganate (KMnO) with a co...

experiment example 1

[0070] The nanoreactor of embodiment 1 and embodiment 2 is detected as follows:

[0071] 1. Particle size and potential: respectively detect the particle size and potential of PTFCG, PTFCG@M, and PTFCG@MH. The measurement method is: take the sample solution and place it in a Marlven Nano ZS instrument, use the dynamic light laser scattering method to detect the particle size, and measure the cell The temperature was set at 25°C, and each sample was operated in triplicate. figure 1 with figure 2 It is the particle size and potential change diagram of PTFCG, PTFCG@M, PTFCG@MH. From the results of the figure, it can be seen that the particle size of PTFCG is 170nm, and the potential is -34mV, and the particle size of PTFCG@M increases to 200nm, the potential rises to +18mV, and continues to wrap HA to form a nanoreactor PTFCG@MH. The particle size increases to 210nm, and the potential drops to -21mV.

[0072] 2. Morphology: observe the shape of PTFCG@MH, the detection method o...

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Abstract

The invention discloses a nanoreactor as well as a preparation method and application thereof. The nanoreactor comprises core-shell nanoparticles, manganese dioxide and a targeting ligand, wherein the surfaces of the core-shell nanoparticles are coated with the manganese dioxide and the targeting ligand layer by layer; the core-shell nanoparticles are formed by a metal organic framework, a photosensitizer, glucose oxidase and a polylactic acid-glycolic acid copolymer nano core wrapped by the metal organic framework, and the metal organic framework is formed by tannic acid and iron through coordination; and the targeting ligand is one or more of hyaluronic acid, synthetic polypeptide, a folic acid modified hydrophilic polymer and a nucleic acid aptamer. The nanoreactor can actively target to tumor cells, catalyzes oxygen production to realize in-situ oxygen supply of tumors, removes glutathione and ATP so as to inhibit and overcome tolerance, and enhances the photodynamic therapy efficiency. The preparation method is simple and effective, effectively avoids the toxicity of a carrier, is high in drug loading capacity and modification rate, and can be applied to preparation of tumor targeting drugs.

Description

technical field [0001] The invention relates to the field of nano-biotechnology, in particular to a nano-reactor and its preparation method and application. Background technique [0002] Photodynamic therapy is a common means of clinical treatment of tumors, however, due to the characteristics of the tumor microenvironment itself, its efficacy is often limited by a variety of resistance mechanisms. For example, tumor hypoxia can directly impair photodynamic therapy efficiency by cutting off oxygen supply and upregulate hypoxia-inducible factor (HIF-1α) leading to tolerance; tumor cell antioxidant mechanisms, such as overexpressed glutathione, can clear The active oxygen generated by photodynamic conversion can eliminate the cell damage caused by active oxygen; tumor cells will spontaneously form an energy-dependent tolerance mechanism, leading to drug efflux and DNA damage repair. Given the complexity of the tumor microenvironment, overcoming multiple resistance mechanisms ...

Claims

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Application Information

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IPC IPC(8): A61K41/00A61K9/51A61K33/32A61K38/44A61K47/34A61P15/14A61P35/00B82Y5/00
CPCA61K9/5161A61K9/5115A61K41/0071A61K33/32A61K47/34A61P35/00A61P15/14A61K38/443C12Y101/03004B82Y5/00A61K2300/00
Inventor 黄术周文虎谢勇
Owner 湖南省人民医院
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