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Method for simultaneously determining 15 fluorescent whitening agents in food contact paper product by high performance liquid chromatography-fluorescence method

A high-performance liquid chromatography, fluorescent whitening agent technology, used in fluorescence/phosphorescence, measuring devices, material analysis by optical means, etc., to achieve good separation and simultaneous detection, fast and efficient sensitivity, and good linear relationship.

Active Publication Date: 2021-05-07
TECH CENT OF GUANGZHOU CUSTOMS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the chromatographic analysis methods of fluorescent whitening agents are usually carried out separately for ionic and non-ionic fluorescent whitening agents, but there are few studies on the simultaneous determination of two types of fluorescent whitening agents

Method used

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  • Method for simultaneously determining 15 fluorescent whitening agents in food contact paper product by high performance liquid chromatography-fluorescence method
  • Method for simultaneously determining 15 fluorescent whitening agents in food contact paper product by high performance liquid chromatography-fluorescence method
  • Method for simultaneously determining 15 fluorescent whitening agents in food contact paper product by high performance liquid chromatography-fluorescence method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1: the selection of chromatographic column

[0030] The 15 kinds of fluorescent whitening agents of the present invention include 6 kinds of ionic and 9 kinds of nonionic fluorescent whitening agents, and there are also some isomers (C.I.220 and C.I.24 and C.I.199A and C.I.199B). The effects of conventional C18 column and Pursuit 5 PFP phenyl column on the simultaneous separation of 15 fluorescent whitening agents were explored. The results show that the C18 column has a good separation degree for ionic C.I.220, C.I.24 and C.I.113, but it is difficult to achieve chromatographic separation for C.I.199A and C.I.199B with isomers and fluorescent whitening agents with similar structures. The Pursuit 5PFP column has a strong retention for ionic fluorescent whitening agents, and the chromatographic separation overlaps, but the chromatographic separation effect for non-ionic fluorescent whitening agents with similar structures is better, so the Pursuit 5 PFP column is...

Embodiment 2

[0031] Embodiment 2: the selection of mobile phase

[0032] Investigated respectively methanol and water, methanol and tetrabutylammonium bromide (TBA) solution and methanol-acetonitrile-TBA solution as mobile phase, the impact on chromatographic separation of 15 kinds of fluorescent whitening agents, such as figure 1 As shown, the results show that tetrabutylammonium bromide (TBA) solution has an important impact on the chromatographic separation of ionic fluorescent whitening agents, and the addition of acetonitrile in the later stage can effectively separate C.I.135 and C.I.199A, so the application adopts three kinds of flow The method of phase mixing realizes effective separation of 15 kinds of fluorescent whitening agents at the same time.

[0033] At the same time, this application has also explored the influence of the mobile phase chromatographic separation under the conditions of pH values ​​of 5, 6, 7, 8 and 9. The results show that the pH value of the TBA solution h...

Embodiment 3

[0034] Embodiment 3: the selection of excitation wavelength and emission wavelength

[0035] By scanning the excitation wavelength, the maximum excitation wavelength is 370nm, and then the emission wavelength is scanned by the maximum excitation wavelength, and the maximum emission wavelength is 440nm. At this time, the fluorescent whitening agent has the best response and high sensitivity.

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Abstract

The invention discloses a method for simultaneously determining 15 fluorescent whitening agents in food contact paper products through a high performance liquid chromatography-fluorescence method, and the 15 fluorescent whitening agents are respectively the fluorescent whitening agents C.I.368, C.I.367, C.I.135, C.I.378, C.I.71, C.I.220, C.I.199A, C.I.24, C.I.134, C.I.113, C.I.199B, C.I.393, C.I.351, C.I.184 and C.I.140. The method has the advantages of being simple and convenient to operate, good in repeatability and high in sensitivity. 15 ionic and non-ionic fluorescent whitening agents which are widely used at present and meet the requirements of laws and regulations and related standards can be measured at the same time.

Description

Technical field: [0001] The invention relates to the technical field of detection, in particular to a method for simultaneously measuring 15 kinds of fluorescent whitening agents in food contact paper products by a high performance liquid chromatography-fluorescence method. Background technique: [0002] Fluorescent whitening agents (FWAs) mostly have conjugated double bond chromophoric groups, which can absorb the invisible ultraviolet rays in sunlight, so that the molecules can be activated and then return to the ground state. In the process, the lower energy The blue and purple light are reflected to offset the yellowish or grayish white in the paper fiber and improve the brightness of the product. Therefore, fluorescent whitening agents are widely used in the paper industry. Some studies have shown that fluorescent whitening agents will have adverse effects on human health. Animal experiments have shown that such substances may have sensitization reactions in direct cont...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/14G01N30/74G01N21/64
CPCG01N30/02G01N30/06G01N30/14G01N30/74G01N21/6428G01N21/643G01N2030/027G01N2030/062G01N2030/146
Inventor 梁瑞钰黎梓城李丹潘静静钟怀宁胨胜
Owner TECH CENT OF GUANGZHOU CUSTOMS
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