Mesenchymal stem cell exosome endometrial preparation, preparation method and uterine cavity perfusion tube

Abstract

The invention discloses a mesenchymal stem cell exosome endometrial preparation, a preparation method and a uterine cavity perfusion tube. The mesenchymal stem cell exosome endometrial preparation is used for prolonging the action time of exosome in a uterine cavity and achieving the purpose of comprehensively treating endometrial injury. The endometrial preparation comprises a mesenchymal stem cell exosome and buffer gel, and the ratio of the mesenchymal stem cell exosome to the buffer gel is (1-5): 1. The preparation method comprises the following steps of acquiring a platelet lysate, namely, collecting whole blood of a patient, carrying out gradient centrifugation to obtain a platelet concentrate, repeatedly freezing and thawing the concentrate, and centrifuging to remove cell debris to obtain the platelet lysate; obtaining the mesenchymal stem cell exosome; and preparing the mesenchymal stem cell exosome endometrial preparation, namely, fully mixing the mesenchymal stem cell exosome with the buffer gel to obtain the mesenchymal stem cell exosome endometrial preparation, and storing the mesenchymal stem cell exosome endometrial preparation at 4 DEG C. The preparation has a good anti-regurgitation effect and can be reserved on the inner wall of the uterine cavity for a long time, so that the repair effect is ensured.

Description

technical field [0001] The invention relates to the technical field of medical devices, in particular to a mesenchymal stem cell exosome endometrial preparation, a preparation method and a uterine cavity perfusion tube. Background technique [0002] The successful implantation of embryos is inseparable from high-quality embryos and good endometrium. Over the past 30 years, with the continuous development of assisted reproductive technology, the embryo culture system has been optimized, the evaluation of embryo selection has been enhanced, and the implantation rate has been further improved. However, according to the latest data research, despite having high-quality embryos for transfer, endometrial damage is still a major bottleneck limiting the increase in pregnancy rate. [0003] Intrauterine perfusion can promote the proliferation of endometrium, which is more conducive to the implantation of fertilized eggs. The time of intrauterine perfusion is generally carried out a...

AI Technical Summary

Problems solved by technology

At present, the infusion of estrogen into the uterine cavity is often used in clinical practice to adjust the thickness of the endometrium. However, there is a rapid reflux of the drug solution out of the uterine cavity, resulting in a short residence time of the drug in the uterine cavity and unable to cover the entire uterine cavity, making it difficult to achieve the desired therapeutic purpose.

Moreover, estrogen has a certain interference effect on endocrine, and there are certain side effects.

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