High-yield bifoliate cremastra appendiculata seedling cultivation method

Abstract

The invention relates to a high-yield bifoliate cremastra appendiculata seedling cultivation method. The high-yield bifoliate cremastra appendiculata seedling cultivation method comprises the following steps of obtaining pure fruit pods of bifoliate cremastra appendiculata, carrying out softening treatment, disinfection and sterilization on testa of the bifoliate cremastra appendiculata to obtain sterile cremastra appendiculata seeds, sowing the sterile cremastra appendiculata seeds into a specific germination culture medium, carrying out germination culture to obtain light yellow protocorms, transferring the protocorms into a proliferation culture medium for proliferation culture to obtain green proliferation protocorms with white villus appearing on the proliferation surface of the green proliferation protocorms, transferring the green proliferation protocorms into a germination culture medium for differentiation germination culture to obtain rootless seedlings with expanded root pseudobulbs and differentiated clustered buds and a pair of leaves, and transferring the seedlings into a rooting culture medium for rooting culture to obtain tissue culture seedlings with expanded root pseudobulbs and 3-5 root hairs. By means of the cultivation method, a large number of high-yield bifoliate cremastra appendiculata domesticated seedlings can be obtained.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for cultivating high-yield rhododendron bilobata seedlings. Background technique [0002] Rhododendron orchid, a perennial orchid herb, the pseudobulb is irregularly oblate or conical, the top gradually protrudes, and the base has fibrous root scars, 1.8 to 3 cm long, and 1 to 2 cm in diameter at the enlarged part. The leaves are usually 1, born on the top of the pseudobulb, narrowly elliptic, subelliptic or oblanceolate narrowly elliptic. Scapes emanate from upper nodes of pseudobulbs, nearly erect, racemes, somewhat drooping, not fully open, fragrant, capsules nearly oval, each containing 1200-2000 tiny seeds. The flowering period is from May to June, and the fruiting period is from September to December. Rhododendron orchid, as a plant in the family Orchidaceae, has many seeds, but the embryo is immature, lacks endosperm, double-layered seed coat, and t...

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Problems solved by technology

The inventor team used this method in practice and found that this method induces rapid germination and high proliferation rate of Rhododendron orchid protocorms, but there are disadvantages such as deformed plant leaves, abnormal pseudobulb expansion, and difficulty in rooting, and it is impossible to obtain a large number of healthy Rhododendron orchid seedlings

[0008] The rhododendron orchid seed germination, multiplication and rooting methods provided by the above-mentioned documents are mainly applicable to the seedling propagation experiment of rhododendron orchid, and further improvement is needed for large-scale rhododendron orchid seedling propagation. Research, including the germination rate of Rhododendron orchid seeds, the proliferation rate of protocorms, and the induced germination rate, all need to be optimized and improved

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