Time-of-flight mass spectrometry multi-target DNA methylation site quantitative detection method
A technology of time-of-flight mass spectrometry and quantitative detection methods, which is applied to biochemical equipment and methods, and microbial measurement/inspection. The effect of cost reduction and accurate results
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[0045] The experimental procedure is attached figure 1 .
[0046] For convenience, the reagents required for the experiment are summarized as follows: EZ DNA Methylation Gold kit (ZYMO), ddH 2 O, PCR Buffer, MgCl 2, dNTPs, Taq Polymerase, SAP Buffer, Shrimp Alkaline Phosphatase Enzyme, iPLEX Buffer Plus, iPLEX Termination Mix, iPLEX Enzyme.
[0047] (1) The detection method of the present invention has a wide range of sample types, such as blood, tissue, cells, etc., and can efficiently extract and prepare genomic DNA for quantitative methylation detection.
[0048]
[0049] (2) CT treatment of DNA:
[0050] I. Reaction system
[0051] Reagent Volume for CT Conversion Reagent ddH2O 9mL M-Dilution Buffer 3mL M-Dissolving Buffer 500μL
[0052] II. Reaction procedure
[0053] temperature time number of cycles 98℃ 10min 1 64℃ 30min 5 4℃ ∞ 1
[0054] (3) Amplify the target fragment:
[0055] I, re...
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