Atlantic salmon DNA bar code standard detection gene, and primer and application thereof

Abstract

The invention relates to an Atlantic salmon DNA bar code standard detection gene, and primers and application thereof, and belongs to the field of molecular biology. The invention also provides a pair of primers for detecting the Atlantic salmon DNA bar code standard detection gene, and application of an Atlantic salmon DNA bar code standard detection sequence in identifying Atlantic salmons. The primers are used for amplifying Atlantic salmon DNA, an obtained PCR amplification product is subjected to nucleotide sequence determination, a species identification result is judged according to a sequence comparison analysis result, and if the genetic distance between the PCR amplification product and a nucleotide sequence shown in SEQ ID NO: 1 is 0.02 or below, it can be judged that a sample to be detected is Atlantic salmon. According to the method, the Atlantic salmons can be distinguished from other salmons and trout types.

Description

technical field [0001] The invention belongs to the field of molecular biology, and in particular relates to an Atlantic salmon DNA barcode standard detection gene, a primer and an application thereof. Background technique [0002] Atlantic Salmon (Salmo salar) belongs to Salmoniformes (Salmoniformes) Salmonidae (Salmonidae) Salmon genus (Salmo), mainly distributed in the North Atlantic Ocean and in the rivers of northern Europe and the east coast of North America. Typical anadromous fish. They live in sea water most of the time, and during the breeding season, they swim to the river where they were born to lay eggs. The young fish will also grow up in the freshwater environment, and then return to the ocean to fatten up. Atlantic salmon is rich in unsaturated fatty acids and astaxanthin, and has extremely high nutritional and health value. It has become an internationally recognized high-end aquatic product and an excellent aquatic product with high development and breedin...

AI Technical Summary

Problems solved by technology

Due to the differences in the meat quality of different species of salmon and trout, their breeding costs, sales prices and nutritional value are also different, and the consumption preferences of different countries are different, resulting in this phenomenon of confusing sales.

For the salmon sold in the market, most of them are muscles, bones and other tissues. There is no complete shape to judge its species, and consumers cannot judge it.

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