Bioreactor for improving antigen yield

Abstract

The invention discloses a bioreactor for improving antigen yield. The bioreactor structurally comprises a reaction tank, a clamping plate, vent holes, an additive pipeline, a motor and an object placing pipe, the surface of the reaction tank is connected with the clamping plate in a welded mode, the clamping plate is in clearance fit with the object placing pipe at the top, the right side of the object placing pipe is in clearance fit with the additive pipeline, and the additive pipeline is connected with a flange at the upper end of the reaction tank. The top of the reaction tank is integrally and movably matched with the motor, the surface of the reaction tank is fixedly connected with the vent holes in an embedded mode, the reaction tank comprises a stirring ring, an oxygen conveying rotating rod, a working cavity, a liquid discharging pipe and an auxiliary rotating frame, and the stirring ring is connected with the surface of the oxygen conveying rotating rod in an embedded mode. Oxygen is supplemented in time while the stirring strip body stirs the interior of the tank, the situation that oxygen floats upwards in the stirring process to cause hypoxia of cells is avoided, the oxygen content is conveyed into the tank in the stirring process under the cooperation of the oxygen dispersing ball, and it is guaranteed that the problems that the oxygen content is too low and the activity of the cells is reduced are solved; on the contrary, the cell activity is improved, and the antigen yield is improved.

Description

technical field [0001] The invention relates to the field of bioreactors, in particular to a bioreactor for improving antigen production. Background technique [0002] Antigen refers to a substance that promotes cell proliferation, differentiation, or produces antibodies and combines with it to exert an immune effect. It is called antigen for short. Antigens generally have two important characteristics, one is immunogenicity, and the other is antigenicity; [0003] However, there are the following deficiencies in the prior art: the current bioreactor for increasing antigen production needs to ensure that the internal carrier protein cells can fully absorb oxygen to enhance vitality during use, but it needs to stir the internal biological cells to make them During the mixing and agitation process, the oxygen at the lower end of the interior will float upwards, causing the internal oxygen to not be supplied to the bottom of the reactor for a while, causing the cell viability a...

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Problems solved by technology

[0004] Aiming at the deficiencies in the prior art, the purpose of the present invention is to provide a bioreactor for increasing the production of antigens, to solve the current problem of a bioreactor for increasing the production of antigens in the prior art, which needs to ensure that the internal carrier protein cells are Sufficiently absorb oxygen to enhance vitality, but it needs to stir to stir the internal biological cells to make them mix. During the stirring process, the internal lower end oxygen will float upwards, causing the internal oxygen to not be supplied to the bottom of the reactor for a while, making the cells at the lower end of the reactor more active. Instantaneous reduction, which in turn causes the problem of reduced antigen production

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