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Establishment and use of carbon tetrachloride induced freshwater fish acute liver and intestine combined injury model

A technology of carbon tetrachloride and freshwater fish, which is applied in application, climate change adaptation, fish farming, etc., can solve the problems of screening and development of immune-enhancing drugs that restrict the mechanism of liver-intestinal damage in fish, and achieve stable and reliable models. good reproducibility

Inactive Publication Date: 2021-04-16
FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in fish, there is no method for constructing a fish liver injury model of oxidative stress, which severely restricts the mechanism of liver-intestinal injury in fish and the screening and development of immune-enhancing drugs.

Method used

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  • Establishment and use of carbon tetrachloride induced freshwater fish acute liver and intestine combined injury model
  • Establishment and use of carbon tetrachloride induced freshwater fish acute liver and intestine combined injury model
  • Establishment and use of carbon tetrachloride induced freshwater fish acute liver and intestine combined injury model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1 A kind of construction method of carbon tetrachloride-induced acute hepatic and intestinal inflammation model of crucian carp, the steps are as follows:

[0029] 1. Transfer the healthy crucian carp with a body weight of (35±3) g into the indoor temperature-controllable circulating water system according to the conventional method, adapt to it for 2 weeks, and feed the basic feed during the adaptation period (the components are: fish meal 8.5%, soybean meal ( 46%) 27.0%, rapeseed meal 17.3%, cotton meal (50%) 13.1%, wheat flour 16.6%, rice bran 10.9%, soybean oil 2.5%, calcium dihydrogen phosphate 1.0%, mineral premix 0.5%, vitamin premix feed 0.5%, vitamin C 0.2%, choline chloride 0.4%, microcrystalline cellulose 1.0%, bentonite 0.5%), breeding conditions are: temperature 28 ± 1 ℃, dissolved oxygen ≥ 6mg / L, pH 6.8-7.5, Ammonia nitrogen content ≤ 0.2mg / L, nitrite content ≤ 0.2mg / L, natural light cycle. A total of 360 domesticated crucian carp were selected...

Embodiment 2

[0045] Embodiment 2: A kind of construction method of carbon tetrachloride-induced crucian carp acute hepatic and intestinal inflammation model, the steps are as follows:

[0046] 1. Transfer the healthy crucian carp with a body weight of (35±3) g into the indoor temperature-controllable circulating water system according to the conventional method, adapt to it for 2 weeks, and feed the basic feed during the adaptation period (the components are: fish meal 8.5%, soybean meal ( 46%) 27.0%, rapeseed meal 17.3%, cotton meal (50%) 13.1%, wheat flour 16.6%, rice bran 10.9%, soybean oil 2.5%, calcium dihydrogen phosphate 1.0%, mineral premix 0.5%, vitamin premix feed 0.5%, vitamin C 0.2%, choline chloride 0.4%, microcrystalline cellulose 1.0%, bentonite 0.5%), breeding conditions are: temperature 28 ± 1 ℃, dissolved oxygen ≥ 6mg / L, pH 6.8-7.5, Ammonia nitrogen content ≤ 0.2mg / L, nitrite content ≤ 0.2mg / L, natural light cycle. A total of 360 domesticated crucian carp were selected a...

Embodiment 3

[0055] Example 3 Screening of hepatoprotective drugs using the carbon tetrachloride-induced acute hepatic and intestinal inflammation model of crucian carp, the steps are as follows:

[0056] 1. According to the conventional method, transfer the healthy pomace bream with a body weight of (24±3) g into the indoor temperature-controllable circulating water system, and adapt to it for 2 weeks. 30.0% soybean meal, 17.8% rapeseed meal, 13.1% cotton meal, 16.6% wheat flour, 10.9% rice bran, 2.5% soybean oil, 1.0% calcium dihydrogen phosphate, 0.5% mineral premix, 0.5% vitamin premix, vitamin C 0.2%, choline chloride 0.4%, microcrystalline cellulose 1.0%, bentonite 0.5%), breeding conditions: temperature 28 ± 1 ℃, dissolved oxygen ≥ 6mg / L, pH 6.8-7.5, ammonia nitrogen content ≤ 0.2mg / L L, nitrite content ≤ 0.2mg / L, natural light cycle.

[0057] 2. Preparation of carbon tetrachloride solution

[0058] Dissolve carbon tetrachloride in sterile olive oil to prepare a 15% carbon tetrach...

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Abstract

The invention relates to an establishment and use of a carbon tetrachloride induced freshwater fish acute liver and intestine combined injury model, and belongs to the technical field of aquaculture. The freshwater fish acute liver and intestine combined injury model is established and induced by adopting carbon tetrachloride. The model is applied to researches of liver and intestine injury mechanisms of fish and screening and development of drugs for liver and intestine protection of fish. The method for inducing the fish liver and intestine injury model in vivo by adopting the carbon tetrachloride solution has a minimum influence on non-specific immunity of fish, and is stable, reliable and good in reproducibility due to the fact that pathological expressions of liver and intestine inflammation are most suitable.

Description

technical field [0001] The invention relates to the establishment and application of a freshwater fish acute hepatic and intestinal combined injury model, belonging to the technical field of aquaculture. Background technique [0002] About carbon tetrachloride (CCl 4 ) mechanism of liver toxicity, there are many hypotheses, but it is unanimously recognized that the main mechanism is the formation of free radicals and the chain peroxidation reaction triggered. CCl 4 In vivo, it can be metabolized and activated by liver microsomal cytochrome P450 to generate two active free radicals (CCl 3 o 2 and Cl) and a series of oxygen reactive substances, which can undergo peroxidation reactions with the membrane lipids of the plasma membrane or subcellular structure of liver cells, and the membrane phospholipids will be degraded in large quantities, thereby destroying the integrity of the cell membrane structure, causing increased membrane permeability, and ultimately leading to live...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/10A01K61/13A61K49/00
CPCY02A40/81
Inventor 缪凌鸿林艳戈贤平刘波
Owner FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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