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Construction method of lipopolysaccharide induced fish multi-organ injury model

A damage model and construction method technology, applied in climate change adaptation, animal husbandry, etc., can solve the problems of fish death, limited application, high mortality, etc., and achieve good reproducibility, stable and reliable models

Active Publication Date: 2020-12-15
FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In many cases, if the fish tissue and organ injury model established by inappropriate methods is used, it is easy to cause too severe damage to the liver, intestine and kidney of the fish, resulting in fish death, high mortality, or too mild damage to the liver, intestine and kidney Capable of gradual self-healing, if the fish organ injury model is unstable, its application is very limited

Method used

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  • Construction method of lipopolysaccharide induced fish multi-organ injury model
  • Construction method of lipopolysaccharide induced fish multi-organ injury model
  • Construction method of lipopolysaccharide induced fish multi-organ injury model

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Experimental program
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Effect test

Embodiment 1

[0027] Embodiment 1 A method for constructing a lipopolysaccharide-induced fish liver injury model, the steps are as follows:

[0028] 1. According to the conventional method, 360 breams with 90-100 g of physical fitness were used as the objects for establishing the organ injury model, and were randomly divided into 6 treatment groups, with 3 repetitions in each group, and 20 tails in each repetition.

[0029] 2. Preparation of lipopolysaccharide solution: dissolve lipopolysaccharide in 0.8% sterile saline solution, and prepare lipopolysaccharides of 0 mg / mL, 1 mg / mL, 2 mg / mL, 3 mg / mL, 4 mg / mL, and 5 mg / mL solution to ensure that the volume of lipopolysaccharide solution or saline solution for injection + intragastric administration of each cultured fish is less than 1mL.

[0030] Among them, the instruments used to configure the lipopolysaccharide solution and the physiological saline must be sterilized and operated on a sterile operating table.

[0031] 3. Establishment of ...

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Abstract

The invention relates to a construction method of a lipopolysaccharide induced fish multi-organ injury model, and belongs to the technical field of aquaculture. The method comprises the following steps of firstly, selecting healthy cultured fishes as objects for establishing an organ injury model; preparing lipopolysaccharide into a lipopolysaccharide solution; carrying out intraperitoneal injection and intragastric administration by adopting the lipopolysaccharide solution to obtain the lipopolysaccharide induced fish organ injury model; and carrying out detection. The construction method ofthe lipopolysaccharide induced fish multi-organ injury model has the minimum influence on the non-specific immunity of the fishes, but the organ injury pathological manifestation of the fishes is mostsuitable, so that the model is stable and reliable, and the reproducibility is good.

Description

technical field [0001] The invention relates to a method for constructing a multi-organ damage model of fish induced by lipopolysaccharide, and belongs to the technical field of aquaculture. Background technique [0002] Lipopolysaccharide, also known as endotoxin, is one of the components of the cell wall of Gram-negative bacteria. It is mainly composed of lipid A, core polysaccharide and O antigen, of which lipid A is the main toxin component of lipopolysaccharide. In some aquatic organisms such as fish, lipopolysaccharide mainly has the functions of immunology, physiology, immunoendocrine and neuroimmune regulation. Low doses of lipopolysaccharide (5, 10, 20 μg / kg) are beneficial to improve the immunity of carp (Cyprinus carpio), however, high doses of lipopolysaccharide (>15 mg / kg) entering cells will be recognized and attacked by the body, stimulating the production of a large number of Biologically active factors participate in the inflammatory response, and the si...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/027
CPCA01K67/027A01K2207/30A01K2227/40A01K2267/03Y02A40/81
Inventor 林艳缪凌鸿陈士友戈贤平刘波
Owner FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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