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Application of MMP19 in serum in anxiety depression

A technology of MMP19, 1. MMP19, applied in the application field of MMP19 in serum in anxiety and depression, can solve the problems related to MMP-19 that have not been reported, and achieve the effect of improving diagnostic efficiency and accuracy

Active Publication Date: 2021-04-13
ACADEMY OF MILITARY MEDICAL SCI +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the relationship between MMP-19 and depression.

Method used

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  • Application of MMP19 in serum in anxiety depression
  • Application of MMP19 in serum in anxiety depression
  • Application of MMP19 in serum in anxiety depression

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Screening markers related to anxiety and depression

[0026] Aiming at young males who faced great mental and psychological pressure during a long-term (7-month) mission, peripheral venous blood was collected before and after the mission (the elbows of all subjects were obtained from the People’s Liberation Army General Hospital from April to November 2012). 5ml of median venous blood, let stand at room temperature for 30min, centrifuge at 1000×g for 15min, aliquot and store in -70℃ refrigerator). Among them, 8 samples were taken before performing the task, and 12 samples were taken after the task. The AAH-BLG-1 chip of Raybiotech Company was used to screen the target protein from 507 factors. The readout is the fluorescence intensity, with higher values ​​indicating higher protein concentrations after normalization. It was found that, compared with pre-task serum, MMP-19 increased significantly in post-task serum (p=0.021458464) ( figure 1 ), intending to ...

Embodiment 2

[0027] Example 2 clinical verification of patients with anxiety and depression

[0028] 1. Sampling

[0029] A total of 19 patients with depression and anxiety syndrome (experimental group) who visited the First Medical Center of the PLA General Hospital from June 2019 to August 2019 were selected. A total of 19 healthy volunteers with normal indicators were collected (control group). Obtain 5ml of cubital median venous blood from all subjects, let it stand at room temperature for 30 minutes, centrifuge at 1000×g for 15 minutes, aliquot and store in a -70 degree refrigerator.

[0030] 2. Experimental process

[0031] The human MMP-19 ELISA kit (purchased from CUSABIO, catalog number CSB-EL014665HU) was selected for the experiment. ELISA operation steps:

[0032] (1) Dilute the capture antibody to the working concentration in PBS, add to the wells of the ELISA plate (100 μl / well), and incubate overnight at room temperature;

[0033] (2) Add 300 μl washing buffer and wash 3...

Embodiment 3

[0048] Example 3 Differential diagnosis

[0049] 1. Sampling

[0050] From June 2020 to October 2020, 15 cases of diabetic peripheral neuropathy (DPN) and 15 cases of cerebral infarction (CI) were treated in the Department of Neurology, Beijing Chaoyang Hospital Affiliated to Capital Medical University. A total of 10 healthy volunteers whose physical examinations were normal in Beijing Chaoyang Hospital were collected. Obtain 5ml of cubital median venous blood from all subjects, let it stand at room temperature for 30 minutes, centrifuge at 1000×g for 15 minutes, aliquot and store in a -70 degree refrigerator.

[0051] 2. ELISA detection

[0052] Experimental procedures refer to the steps described in Example 2.

[0053] 3. Experimental results

[0054] The result is as image 3 As shown, there was no difference in serum MMP19 level between diabetic peripheral neuropathy (DPN) and normal people (P=0.5417), and there was no difference between cerebral infarction (CI) and con...

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Abstract

The invention relates to the field of biological detection, in particular to application of MMP19 in serum in anxiety depression. The invention provides an application of MMP19 protein or a specific antibody thereof in preparation of a product for detecting or diagnosing anxiety depression. According to the invention, serum MMP19 protein is used as a new marker for anxiety depression screening drugs and diagnosis. A kit for diagnosing the anxiety depression is prepared for the first time by aiming at the MMP19, the anxiety depression can be screened and diagnosed only by detecting the concentration of the MMP-19 in serum of a sample, the diagnosis efficiency and accuracy are improved, and an important value is provided for early diagnosis of the anxiety depression. Great help is provided for the prevention and treatment of anxiety and depression in China.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to the application of serum MMP19 in anxiety and depression. Background technique [0002] Depression belongs to the category of affective disorders, which is a mood disorder or affective disorder caused by a variety of factors. Depressed mood, slowed thinking, decreased volitional activity, impaired cognitive function, loss of interest in activities and decreased ability, loss of self-esteem, inappropriate feelings of guilt, thoughts of death and suicide, decreased concentration, sleep disturbance, and decreased appetite , loss of libido, can also be accompanied by various physical symptoms. [0003] Our country is in a transitional period of rapid development, the living and working pressure of urban and rural residents is increasing, and social psychological problems are becoming more and more prominent. According to the survey report of the World Health Organization, about ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/573
CPCG01N33/573G01N2333/96494G01N2800/301G01N2800/304
Inventor 张纪岩杨锡琴何有娣石强董洁程倩倩牛春晓何佳
Owner ACADEMY OF MILITARY MEDICAL SCI
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