Method for detecting glutathione by using fluorescence enhancement method

A glutathione and fluorescence enhancement technology, applied in fluorescence/phosphorescence, measuring devices, and material analysis through optical means, can solve problems such as patent publications that have not yet been found, and achieve wide detection linear range, enhanced fluorescence intensity, good selective effect

Inactive Publication Date: 2021-04-13
TIANJIN UNIV OF SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Through the search, no patent publications related to the patent application of the present invention have been found

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  • Method for detecting glutathione by using fluorescence enhancement method
  • Method for detecting glutathione by using fluorescence enhancement method
  • Method for detecting glutathione by using fluorescence enhancement method

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Embodiment Construction

[0032] The embodiments of the present invention will be described in detail below. It should be noted that the embodiments are illustrative, not restrictive, and cannot limit the protection scope of the present invention.

[0033] The raw materials used in the present invention, unless otherwise specified, are conventional commercially available products; the methods used in the present invention, unless otherwise specified, are conventional methods in the art.

[0034] A method utilizing fluorescence enhancement method to detect glutathione, the steps are as follows:

[0035] ⑴ Put the fluorescent material and glutathione in water, mix well, the final concentration of fluorescent material is 0.001-0.1mg·mL -1 , the final concentration of glutathione was 6.0×10 -10 -1.0×10 -3 mg mL -1 ;

[0036] (2) Add surfactants, cations and anions to the above mixed solution to form a system for fluorescent detection of glutathione; wherein, the mass final concentration of surfactants is...

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Abstract

The invention relates to a method for detecting glutathione by using a fluorescence enhancement method, which comprises the following steps: (1), putting a fluorescent material and glutathione into water, and uniformly mixing the mixture; (2), adding a surfactant, cations and anions into the mixed solution to form a glutathione fluorescence detection system; (3), uniformly mixing the prepared fluorescence detection system, and placing the mixture in an environment of 20-40 DEG C for detection; and (4), irradiating the fluorescence detection system by adopting 980nm excitation, and recording a fluorescence intensity F value at 550nm to obtain a product. According to the method, the characteristic that the up-conversion nano material can emit visible light under the excitation of near-infrared light is utilized, and the fluorescence of the up-conversion nano material can be enhanced in a system containing a surfactant, cations and anions, so that glutathione in the system is detected by high-sensitivity sensing, and the method is high in selectivity and good in stability.

Description

technical field [0001] The invention belongs to the technical field of detection of small molecule biological thiol compounds, in particular to a method for detecting glutathione by using a fluorescence enhancement method. Background technique [0002] Glutathione is the most important non-protein thiol compound in living cells of mammals and eukaryotic cells. It is an important endogenous antioxidant and antidote, and plays an important role in scavenging free radicals in biological systems. Glutathione is the first line of defense against toxic substances, and can be widely involved in human immune regulation, metabolism, energy transportation and other physiological processes. It plays a key role in maintaining cellular redox homeostasis, cell signal transduction, and detoxification. Generally, the imbalance of glutathione concentration in the human body can lead to many diseases, such as white blood cell loss, psoriasis, liver damage, cancer, human immunodeficiency viru...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6402G01N21/6486
Inventor 邓启良孙蕊袁渠淋
Owner TIANJIN UNIV OF SCI & TECH
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