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Serum 18 type riemerella anatipestifer and application

A technology of Riemerella anatipestifer and Merella anatipestifer, applied to bacteria, vaccines, antibacterial drugs, etc., to achieve strong pathogenicity, stable biological characteristics, and reduced morbidity and mortality

Active Publication Date: 2021-04-13
重庆永健生物技术有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the diversity and variability of antigens in RA, there are many types and complex serotypes, but no recognized serotype 18 Riemerella anatipestifer has been reported yet.

Method used

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  • Serum 18 type riemerella anatipestifer and application
  • Serum 18 type riemerella anatipestifer and application
  • Serum 18 type riemerella anatipestifer and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1 Isolation and identification of serotype 18 Riemerella anatipestifer

[0017] 1. Materials

[0018] 1.1 Serum

[0019] Rabbit positive serum against Riemerella anatipestifer type 1 and 2, donated by the Infectious Disease Laboratory of the School of Veterinary Medicine, China Agricultural University;

[0020] Riemerella anatipestifer type 1, 2, 3, 4, 5, 6, 7, 8, 10, 11 positive serum, self-made.

[0021] 1.2 Strains to be tested

[0022] A suspected strain isolated from dead ducks suspected to be infected with Riemeria anatipestifer in Shanghai area.

[0023] 1.3 Known serotype strains

[0024] Serotype 1 and 2 strains of Riemeria anatipestifer (also called Riemerella anatipestifer type 1 and Riemerella anatipestifer type 2) were purchased from China Veterinary Drug Control Institute;

[0025] Riemerella anatipestifer serotypes 3, 4, 5, 6, 7, 8, 10, and 11 were all donated by the Infection Laboratory of the School of Veterinary Medicine, China Agricultura...

Embodiment 2

[0067] Example 2 Toxicity study of serotype 18 Riemerella anatipestifer

[0068] 1. Materials

[0069] 1.1 Strains

[0070] Riemerella anatipestifer type 1 strain (YJ821-1), type 2 strain (YJ821-2), type 18 strain (YJ821-18).

[0071] 1.2 Medium

[0072] Tryptic Soy Agar (TSA) with 5% Calf Serum

[0073] 1.3 Test animals

[0074] Healthy ducklings whose serum antibodies to Riemeria anatipestifer were negative for RA.

[0075] 2. Method

[0076] 2.1 Bacteria culture

[0077] Take one basal strain of F2 generation Riemeria anatipestifer preserved in liquid nitrogen, streak on a TSA plate, and store at 37°C, 5% CO 2 Cultivate in an incubator for 24 to 48 hours, pick typical colonies and inoculate them on a TSA plate, at 37°C, 5% CO 2 Cultivate in an incubator for 24 to 48 hours, wash the bacterial lawn with 30% sterilized glycerol saline, adjust the count of viable bacteria by turbidimetric method, and freeze at -80°C after aliquoting.

[0078] 2.2 Bacterial liquid inspe...

Embodiment 3

[0146] Example 3 Toxicity study of serotype 18 Riemerella anatipestifer

[0147] Three strains of type 1, two strains of type 2, and two strains of type 18 used in the animal regression test all had good virulence, and the same RA as the challenger bacteria could be isolated from the dead ducks challenged with the virus. The immunogenicity of these strains of RA is now explored.

[0148] 1. Materials

[0149] 1.1 Strains

[0150] Three strains of Riemerella anatipestifer type 1, tentatively named RA1-1, RA1-2, RA1-3; two strains of type 2, tentatively named RA2-1, RA2-2; two strains of type 18, tentatively named RA18-1, RA18-2.

[0151] 1.2 Medium

[0152] Tryptic Soy Agar (TSA) with 5% Calf Serum

[0153] 1.3 Test animals

[0154] Healthy ducklings tested negative for serum antibodies.

[0155] 2. Method

[0156] 2.1 Bacteria culture

[0157] Pick the strains and inoculate them on TSA, incubate in a 37°C incubator for 24-48 hours to form colonies, wash the bacterial ...

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PUM

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Abstract

The invention provides serum 18 type riemerella anatipestifer and application. The strain is named as riemerella anatipestifer YJ821-18, has a preservation number of CCTCC NO: M2020528, and is preserved in CCTCC on September 21, 2020. The strain has stable biological characteristics, relatively strong pathogenicity to ducklings and good immunogenicity. An oil adjuvant inactivated vaccine prepared from the strain is safe and reliable, can generate relatively high-level antibodies, is long in lasting period and has a very good protection effect on ducks attacked by homologous strains, the morbidity and mortality of immunized ducks are obviously reduced, and the prevalence of riemerella anatipestifer diseases can be effectively prevented.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products, and in particular relates to a serotype 18 Riemerella anatipestifer and its application. Background technique [0002] Riemerella anatipestifer, also known as duck infectious serositis, new duck disease, and duck plague syndrome, is a contact acute or chronic septicemia caused by Riemerella anatipestifer (RA). Infectious disease, mainly affects ducklings aged 2-7 weeks. Clinically, it mainly manifests as increased eye and nasal secretions, panting, coughing, diarrhea, ataxia and head and neck tremor. The diseased ducks that pass through chronically mainly manifest as meningitis Symptoms, neck tilt, slow growth or stiff ducks, lesions are characterized by fibrinous pericarditis, perihepatitis and air sacs, and the mortality rate is as high as 90%, causing huge economic losses to the duck industry. The survey shows that Riemerella anatipestifer has occurred in all parts of o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K39/02A61P31/04C12R1/01
CPCC12N1/20A61K39/0208A61P31/04A61K2039/552A61K2039/521Y02A50/30
Inventor 何信群李来旭苏敬良刘哲君徐光菊李阁张强王灵强田燕
Owner 重庆永健生物技术有限责任公司
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