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Extraction method of callicarpa kwangtungensis polysaccharide

An extraction method and technology of Zizhu, applied in the field of plant extraction and analysis, can solve the problems of difficult to achieve efficient application of Guangdong Zizhu polysaccharide, complex components, and many polysaccharide impurities.

Inactive Publication Date: 2021-04-13
HENAN POLYTECHNIC UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the prior art, the basic research on the pharmacodynamic substances of Guangdong Zizhu mainly focuses on the small molecular chemical components that have been discovered. With the deepening of research, the research on macromolecules in Guangdong Zizhu has gradually received attention, including Extraction of water-soluble polysaccharides, but the polysaccharides extracted by the methods of the prior art have many impurities and complex components, and it is difficult to realize the efficient application of Guangdong Zizhu polysaccharides

Method used

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  • Extraction method of callicarpa kwangtungensis polysaccharide
  • Extraction method of callicarpa kwangtungensis polysaccharide

Examples

Experimental program
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Effect test

Embodiment 1

[0027] A method for extracting Guangdong Zizhu polysaccharide, comprising the steps of:

[0028] (1) Guangdong Zizhu uses the microwave alcohol extraction method to extract the active ingredients. After the Guangdong Zizhu is washed, dried and crushed, add 4 times the volume of 70wt% ethanol solution, reflux for 2 hours, and microwave under the condition of power 150W Continue heating for 15 minutes, cool to room temperature and concentrate under reduced pressure to obtain the extract:

[0029] (2) Separation of macromolecules:

[0030] Component A of natural clarifier: Dissolve Type II ZTC1+1 natural clarifier in distilled water to make 1wt% solution A;

[0031] Natural clarifier B component: Dissolve Type II ZTC1+1 natural clarifier in 1wt% glacial acetic acid solution to make 1wt% solution B;

[0032] Dissolve the extract of step (1) in water to make a 1wt% solution, and at 90°C, add solution B dropwise at a volume ratio of 100:3, mix for 1 hour, then add solution A dropw...

Embodiment 2

[0038] A method for extracting Guangdong Zizhu polysaccharide, comprising the steps of:

[0039] (1) Guangdong Zizhu uses the microwave alcohol extraction method to extract the active ingredients. After the Guangdong Zizhu is washed, dried and crushed, it is added to 5 times the volume of 60wt% ethanol solution, and then refluxed for 2.5 hours, under the condition of power 200W Microwave heating was continued for 10 minutes, cooled to room temperature and then concentrated under reduced pressure to obtain the extract:

[0040] (2) Separation of macromolecules:

[0041] Natural clarifier A component: the clarifier KBT-ZTC is dissolved in distilled water to make 1wt% solution A;

[0042] Component B of natural clarifier: dissolve clarifier KBT-ZTC in 1wt% glacial acetic acid solution to make 1wt% solution B;

[0043] Dissolve the extract of step (1) in water to make a 1.5wt% solution, at 100°C, add solution B dropwise at a volume ratio of 100:2.5, mix for 1.5 hours, then add s...

Embodiment 3

[0049] A method for extracting Guangdong Zizhu polysaccharide, comprising the steps of:

[0050] (1) Guangdong Zizhu uses the microwave alcohol extraction method to extract the active ingredients. After the Guangdong Zizhu is washed, dried and crushed, it is added to 6 times the volume of 50wt% ethanol solution, refluxed for 3 hours, and microwaved at a power of 250W. Continue heating for 5 minutes, cool to room temperature and concentrate under reduced pressure to obtain the extract:

[0051] (2) Separation of macromolecules:

[0052] Component A of natural clarifier: Dissolve Type II ZTC1+1 natural clarifier in distilled water to make 1wt% solution A;

[0053] Natural clarifier B component: Dissolve Type II ZTC1+1 natural clarifier in 1wt% glacial acetic acid solution to make 1wt% solution B;

[0054] Dissolve the extract of step (1) in water to make a 2wt% solution, and at 110°C, add solution B dropwise at a volume ratio of 100:2, mix for 2 hours, then add solution A dropwi...

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Abstract

The invention relates to the technical field of plant extraction and analysis, in particular to an extraction method of callicarpa kwangtungensis polysaccharides, which comprises the following steps: extracting effective components from callicarpa kwangtungensis by a microwave alcohol extraction method, separating macromolecules, continuing high-performance counter-current chromatography separation, and finally obtaining the callicarpa kwangtungensis polysaccharides by a column chromatography elution separation technique. The method comprises the following steps: extracting effective components to obtain an extract, removing colloid unstable components such as tannin, protein, resin, wax paper and the like in the extract, carrying out high performance countercurrent chromatography treatment, and removing water-insoluble organic matters, so that the retained water-soluble substances contain water-soluble polysaccharides, water-soluble amino acids and polypeptides; and finally, effectively separating the callicarpa kwangtungensis polysaccharide by adopting a column chromatography elution separation technology to obtain the callicarpa kwangtungensis polysaccharide with high purity.

Description

technical field [0001] The invention relates to the technical field of plant extraction and analysis, in particular to a method for extracting Guangdong purple pearl polysaccharide. Background technique [0002] Guangdong Zizhu is a deciduous shrub belonging to Verbenaceae and Zizhu, about 2 meters high; the leaves are ovate-elliptic to elliptic, with long acuminate to short pointed tips, wedge-shaped bases, fine serrations on the edges, grayish brown on the back, and two sides Dense dark red or red granular glandular spots, distributed in Henan, Jiangsu, Anhui, Zhejiang, Jiangxi, Hunan, Hubei , Guangdong, Guangxi, Sichuan, Guizhou, Yunnan and other places and Vietnam; born in forests, forest margins and shrubs at an altitude of 200-2300 meters, the root or whole plant of this species is used as medicine, which can stimulate menstruation and blood, and treat irregular menstruation Diarrhea, asthenia, leucorrhea, postpartum blood gas pain, cold and cold embolism. [0003] I...

Claims

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Application Information

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IPC IPC(8): C08B37/00
CPCC08B37/0003C08B37/006
Inventor 王振辉牛超张真真杨兰萍孔继川周德军翟洋洋
Owner HENAN POLYTECHNIC UNIV
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