Preparation method of bacteriostatic household paper
A technology of tissue paper and antibacterial zone, applied in the preparation of test samples, methods based on microorganisms, biochemical equipment and methods, etc. The bacteria effect is not stable and other problems, to achieve the effect of convenient measurement, fast and intuitive acquisition, absorption and distribution
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Embodiment 1
[0025]A method of preparation of antibacterial living paper, including the following steps:
[0026]Step 1: Get a paper sample equal to 1 cm in diameter;
[0027]Step 2: Put the paper sample acquired by step into the test tube to close the sterilization treatment, the sterilization temperature is 121 ° C, the sterilization time is 20 min;
[0028]Step 3: Put the paper samples after step dilapidate into the benzalkammonium bromide solution, and remove the extracted paper sample to remove the excess benzalkonium bromide solution to dry treatment, and The concentration of the benzalkonium bromide solution is 0%;
[0029]Step 4: Top-dried paper samples for ultraviolet disinfection, the disinfection time is 30 min;
[0030]Step 5: Put the sterilized medium into a petri dish having a thickness of 4 mm, and then add E. coli suspension into the petri dish, and obtain uniform coating of Escherichia coli suspension in the medium by turning the petri dish. Coated medium with Escherichia coli suspension, wher...
Embodiment 2
[0034]A method of preparation of antibacterial living paper, including the following steps:
[0035]Step 1: Get a paper sample equal to 1 cm in diameter;
[0036]Step 2: Put the paper sample acquired by the step into the test tube to close the sterilization, the sterilization temperature is 120 ° C, and the sterilization is 25 min;
[0037]Step 3: Put the paper samples after step dilapidate into the benzalkammonium bromide solution, and remove the extracted paper sample to remove the excess benzalkonium bromide solution to dry treatment, and The concentration of the benzaplammonium bromide solution is 0.13%;
[0038]Step 4: Top-dried paper samples were used for ultraviolet disinfection, and the disinfection time was 35min;
[0039]Step 5: Put the sterilized medium into a petri dish having a thickness of 4 mm, and then add E. coli suspension into the petri dish, and obtain uniform coating of Escherichia coli suspension in the medium by turning the petri dish. Coated medium with Escherichia coli sus...
Embodiment 3
[0043]A method of preparation of antibacterial living paper, including the following steps:
[0044]Step 1: Get a paper sample equal to 1 cm in diameter;
[0045]Step 2: Put the paper sample acquired by step into the test tube to close the sterilization treatment, the sterilization temperature is 122 ° C, the sterilization time is 23 min;
[0046]Step 3: Put the paper samples after step dilapidate into the benzalkammonium bromide solution, and remove the extracted paper sample to remove the excess benzalkonium bromide solution to dry treatment, and The concentration of the benzalkonium bromide solution is 0.25%;
[0047]Step 4: Top-dried paper samples were used for ultraviolet disinfection, and the disinfection time was 35min;
[0048]Step 5: Put the sterilized medium into a petri dish having a thickness of 4 mm, and then add E. coli suspension into the petri dish, and obtain uniform coating of Escherichia coli suspension in the medium by turning the petri dish. Coated medium with Escherichia coli...
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