Myelin oligodendrocyte glycoprotein antibody kit and detection method thereof

A technology of oligodendrocytes and glycoproteins, which is applied in the fields of peptide chemistry and immunology, can solve the problems affecting the treatment process and elevation of patients, and achieve the effects of high specificity, good sensitivity, good detection accuracy and precision

Active Publication Date: 2021-04-06
安徽恩禾生物技术有限公司
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0004] The amount of MOG-Ab (anti-MOG antibody) in the blood of patients with MOG-Ab disease will increase, and the existing detectio

Method used

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  • Myelin oligodendrocyte glycoprotein antibody kit and detection method thereof
  • Myelin oligodendrocyte glycoprotein antibody kit and detection method thereof
  • Myelin oligodendrocyte glycoprotein antibody kit and detection method thereof

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Embodiment Construction

[0028] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0029] A myelin oligodendrocyte glycoprotein antibody kit, the kit uses a double-antigen sandwich method to detect the human MOG antibody, the composition of the kit is as follows:

[0030] Pre-coated plates: 48 / 96 wells

[0031] MOG-Ab calibrator: 5 pieces, 5×1ml (concentrations are 0ug / ml, 0.5ug / ml, 1ug / ml, 2ug / ml, 5ug / ml, 10ug / ml)

[0032] MOG-Ab quality control product: 1 piece, derived from human serum, the quality control range is 7±1.05ug / ml

[0033] ...

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Abstract

The invention belongs to the technical field of polypeptide chemistry and immunology, and particularly relates to a myelin oligodendrocyte glycoprotein antibody kit, which takes recombinant protein coded by an extracellular region sequence of a human myelin oligodendrocyte glycoprotein gene as a coating antigen. The invention also provides a detection method of the kit. The method comprises the following steps: separating to obtain an antigen-to-be-detected antibody-binding antigen-MOG enzyme conjugate compound, adding a color developing agent A and a color developing agent B, adding a stop solution, detecting by using a microplate reader, and calculating the concentration of MOG-Ab according to the absorbance OD value and a standard curve. According to the invention, the defects in the prior art are overcome,the myelin oligodendrocyte glycoprotein antibody in serum can be quickly and specifically detected, and the kit is good in reaction specificity, high in sensitivity, simple to operate, low in cost, stable and reliable in reaction result and easy to observe.

Description

technical field [0001] The invention belongs to the technical field of polypeptide chemistry and immunology, and in particular relates to a myelin oligodendrocyte glycoprotein antibody kit and a detection method thereof. Background technique [0002] Myelin oligodendrocyte glycoprotein (MOG), a member of the immunoglobulin superfamily, consists of 218 amino acids and is a component of myelin in the central nervous system. MOG is expressed relatively late in the development of the nervous system, which suggests that it may be an important surface marker of oligodendrocyte maturation, and it plays an important role in maintaining the integrity of myelin sheath and promoting the exchange of information between cells. Current studies have shown that anti-MOG antibodies may be the cause of immune demyelinating diseases of the central nervous system, such as acute disseminated encephalomyelitis (ADEM), multiphasic disseminated encephalomyelitis (MDEM), optic neuritis (ON), long-te...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/564G01N33/535
CPCG01N33/6893G01N33/564G01N33/535G01N2800/285
Inventor 余旭亮金腾川郝洪军
Owner 安徽恩禾生物技术有限公司
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