Novel nucleic acid extraction-free preservation solution

Abstract

The invention relates to a novel nucleic acid extraction-free preservation solution, and discloses the preservation solution capable of being directly used for detection without subsequent nucleic acid extraction and a preparation method of the preservation solution. The preservation solution comprises the following components: 0.1-2% of a surfactant, 0.1-2% of an acid-base buffer agent, 1-5% of citrate, 0.1-5% of weak acid, 0-10% of guanidine salt, 0-0.1% of protease K, 0.1-1% of a chelating agent. 0.1-2% of an antiseptic agent, with the balance being water. The preservation solution is used for preserving tissue and cell analysis samples, and comprises coronaviruses, influenza viruses, hand-foot-and-mouth viruses, measles rubella viruses and other types of viruses, mycoplasma and chlamydia. After a nasal swab and a pharynx swab are placed in the preservation solution, the unique components of the preservation solution can protect nucleic acid RNA from being degraded by RNA enzyme and protect deoxyribonucleic acid DNA from being degraded by DNA enzyme; and the preservation solution has strong antibacterial and antifungal effects through combined use of multiple preservatives.

Description

Technical field: [0001] The invention belongs to the technical field of molecular biology, and specifically relates to a novel preservation solution that can be directly used for detection without subsequent nucleic acid extraction and a preparation method thereof. Background technique: [0002] Nucleic acid refers to the general term of deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). It is a biological macromolecular polymer polymerized by many nucleotide monomers. It is one of the most basic substances of life and is all known forms of life. The essential constituent substances are the most important substances in all biomolecules, and are widely present in all animal and plant cells and microorganisms. Nucleic acid is composed of nucleotides, and nucleotide monomers are composed of five-carbon sugars, phosphate groups and containing Nitrogen bases; if the five-carbon sugar is ribose, the polymer formed is RNA; if the five-carbon sugar is deoxyribose, the polymer f...

AI Technical Summary

Problems solved by technology

[0003] For the preservation of viruses, the traditional methods mostly use common ingredients such as phosphate and sodium chloride. The disadvantage is that they can only be used for preservation, and the nucleic acid still needs to be extracted again before subsequent detection, which is one of the traditional preservation-extraction-detection steps. First, the operation is troublesome

The invention patent "A Virus Preservation Solution Free of Nucleic Acid Extraction (Application No. 202010148767.4)" discloses a virus preservation solution free of nucleic acid extraction. Although it can be used for virus preservation, it has the following disadvantages: 1. Tween 20 is more prone to foaming , due to the small volume of the general sampling tube, the foam is easy to overflow outside the tube and cause cross-infection. At the same time, Tween 20 affects the appearance and light transmittance of the solution; 2. Acid protease is more expensive and difficult to popularize in large areas

The invention patent "A Nucleic Acid Extraction Free Reagent and Its Application Method (Application No. 202011191934.X)" discloses a nucleic acid extraction free reagent technology, but has the following disadvantages: 1. Chelex-100 and digitonin are relatively expensive and Not easy to obtain; 2. There is only a single buffer of Tris-Hcl, and the buffering capacity is not strong

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