Preparation method of L-proline

Abstract

The invention discloses a preparation method of Lproline, and the method comprises the following steps: 1, filtering proline fermentation liquor through a filter membrane to obtain ultrafiltration filtrate, and cleaning with pure water to obtain microfiltration filtrate; 2, enabling the micro-filtration clear liquid to sequentially pass through weakly-alkaline anion resin and weakly-acidic cation resin, and performing filtering to obtain filtrate; 3, adding activated carbon into the filtrate for decolorization, and performing filtering to obtain a decolorized solution containing proline; 4, evaporating and concentrating the decolorized solution to obtain a concentrated solution, wherein the content of the concentrated solution is 45-50%; 5, cooling the concentrated solution to 10 DEG C, performing natural crystallizing, and performing centrifuging to obtain wet crystals; 6, putting the wet crystals into a vacuum drying machine for drying to obtain a finished product of Lproline. The method for preparing the Lproline is relatively simple in process control, does not generate a large amount of pollutants in the production process, and is environment-friendly.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a preparation method of L-proline. Background technique [0002] L-proline (referred to as proline) is one of the 20 kinds of amino acids synthesized by the human body. It is a colorless to white crystal or crystalline powder at room temperature. Insoluble in ether, butanol and isopropanol, soluble in mineral acids. Amino acid is a general term for a class of organic compounds containing amino and carboxyl groups. The presence of amino acids in the human body not only provides important raw materials for protein synthesis, but also provides a material basis for promoting growth, normal metabolism, and maintaining life. [0003] At present, the methods for producing hydroxyproline reported at home and abroad mainly include proteolytic extraction, chemical synthesis, microbial fermentation and enzymatic conversion. Among them, proteolytic extraction is currently the most commonly used...

AI Technical Summary

Problems solved by technology

The subsequent treatment process is long, which increases the discharge of three wastes and causes environmental pollution. At the same time, due to the large amount of impurities in the material, the light transmittance of the product after ion exchange is low, which affects product quality.