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Method for rapidly separating triterpenes and sterols in lucid ganoderma alcohol extract

A technology for Ganoderma lucidum alcohol and sterol, which is applied in the field of extraction and separation of natural medicines, can solve the problems of complicated process, difficult separation of Ganoderma lucidum triterpenes and sterols, and high production cost, and achieves the effects of high separation accuracy, low cost and easy operation.

Active Publication Date: 2021-04-06
GUANGZHOU HANFANG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a simple and quick method for the prior art to separate ganoderma triterpenoids and sterols, which are difficult to separate from ganoderma lucidum and sterols, and the process is cumbersome and high. Sterols

Method used

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  • Method for rapidly separating triterpenes and sterols in lucid ganoderma alcohol extract
  • Method for rapidly separating triterpenes and sterols in lucid ganoderma alcohol extract
  • Method for rapidly separating triterpenes and sterols in lucid ganoderma alcohol extract

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Experimental program
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Effect test

Embodiment 1

[0032] S1. Take an appropriate amount of polyamide resin (100-200 mesh), add ethanol to immerse, remove air bubbles by ultrasonication for 10 minutes, and let stand for 10 hours after stirring;

[0033] S2. Column packing: select a glass chromatography column with a column diameter of 2.6cm, fill it to a column height of 20cm, and a column diameter ratio of 1:7, and elute with deionized water until there is no alcohol smell;

[0034] S3. Take 1.0 g of the ethanol extract of Ganoderma lucidum fruiting body, mix the sample with the polyamide resin soaked in step S1 according to the mass ratio of 1:1, evaporate the ethanol in an evaporating dish, and then load the sample;

[0035] S4. First wash the column with 50% ethanol for 3 times the column volume to obtain eluent A; then wash the column with 90% ethanol for 3 times the column volume to obtain eluent B;

[0036] S5, carry out chromatographic detection to eluent A and eluent B, the result is as follows figure 1 shown, from ...

Embodiment 2

[0039] S1. Take an appropriate amount of polyamide resin (100-200 mesh), add ethanol to immerse, remove air bubbles by ultrasonication for 10 minutes, and let stand for 15 hours after stirring;

[0040] S2. Column packing: select a glass chromatography column with a column diameter of 5.5 cm, fill it to a column height of 30 cm, and a column diameter ratio of 1:5, and elute with deionized water until there is no alcohol smell;

[0041] S3. Take 5.0 g of the ethanol extract of Ganoderma lucidum fruiting body, mix the sample with the polyamide resin soaked in step S1 according to the mass ratio of 1:1.5, evaporate the ethanol to dryness in an evaporating dish, and then load the sample;

[0042] S4. First wash the column with 55% ethanol for 5 times the column volume to obtain eluent A; then wash the column with 85% ethanol for 5 times the column volume to obtain eluent B;

[0043] S5, carry out chromatographic detection to eluent A and eluent B, the result is as follows figure...

Embodiment 3

[0046] S1. Take an appropriate amount of polyamide resin (100-200 mesh), add ethanol to immerse, stir to remove air bubbles, and let stand for 12 hours after stirring;

[0047] S2. Column packing: select a glass chromatography column with a column diameter of 6cm, fill it to a column height of 36cm, and a column diameter ratio of 1:6, and elute with deionized water until there is no alcohol smell;

[0048] S3. Take 10.0 g of the ethanol extract of Ganoderma lucidum fruiting body, mix the sample with the polyamide resin soaked in step S1 according to the mass ratio of 1:1, evaporate the ethanol to dryness in an evaporating dish, and then load the sample;

[0049] S4. First wash the column with 45% ethanol for 3 times the column volume to obtain eluent A; then wash the column with 80% ethanol for 3 times the column volume to obtain eluent B;

[0050] S5, carry out chromatographic detection to eluent A and eluent B, the result is as follows image 3 shown, from image 3 It can be...

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Abstract

The invention relates to the field of extraction and separation of natural medicines, and discloses a method for rapidly separating triterpenes and sterols in a lucid ganoderma alcohol extract. The method comprises the following steps: packing a column with polyamide resin, eluting with a solvent in a specific ratio to obtain triterpene and sterol eluent in the ganoderma lucidum alcohol extract, and concentrating and drying the eluent to obtain ganoderma lucidum triterpene and ganoderma lucidum sterol. Ganoderma lucidum sterol and ganoderma lucidum triterpenes are similar in structure, similar in polarity, easy to dissolve in an organic solvent, high in separation difficulty, complex in traditional preparation process and high in production cost. Aiming at the defects in the prior art, the invention provides the method for rapidly separating the ganoderma triterpene and sterol components, and the method is very simple and convenient to operate, high in separation accuracy, good in reproducibility, high in safety, lower in cost and suitable for industrial production.

Description

technical field [0001] The invention relates to the field of extraction and separation of natural medicines, in particular to a method for rapidly separating triterpenes and sterols in ganoderma lucidum ethanol extract. Background technique [0002] Ganoderma lucidum (Ganoderma lucidum) is a precious medicinal fungus, belonging to the Basidiomycetes polyporaceae Ganoderma lucidum genus Ganoderma and Zizhi. It was first recorded in "Shen Nong's Herbal Classic". Consolidate, prolong life and other effects. Ganoderma lucidum is rich in components, including polysaccharides, triterpenes and sterols, nucleosides, proteins and trace elements, among which triterpenes, sterols and polysaccharides of Ganoderma are the most important active substances. Modern medical research on Ganoderma lucidum and its extracts found that Ganoderma lucidum triterpenoids have anti-tumor, liver protection, hypoglycemic, angiotensin-inhibiting, and anti-oxidative effects; Cholesterol concentration, p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/074A61P35/00A61P1/16A61P3/10A61P37/04A61P3/06A61P13/08
CPCA61K36/074A61P35/00A61P1/16A61P3/10A61P37/04A61P3/06A61P13/08A61K2236/55
Inventor 蔡鸿飞张琴许文东袁诚刘菊妍杨阳刘春芳
Owner GUANGZHOU HANFANG PHARMA
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