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Method for rapidly detecting 12 respiratory viruses by using multiplex PCR technology and application thereof

A respiratory and multiple technology, applied in the field of genetic engineering, can solve the problems of difficult biological classification and poor specificity of 12 respiratory viruses, and achieve the effect of high specificity and good conservation.

Pending Publication Date: 2021-03-26
哈尔滨星云医学检验所有限公司
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Problems solved by technology

[0003] The present invention provides a method for rapidly detecting 12 kinds of respiratory viruses by using multiplex PCR technology in order to solve the difficult problem of biological classification of 12 kinds of respiratory viruses and poor specificity. The method comprises the following steps:

Method used

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  • Method for rapidly detecting 12 respiratory viruses by using multiplex PCR technology and application thereof
  • Method for rapidly detecting 12 respiratory viruses by using multiplex PCR technology and application thereof
  • Method for rapidly detecting 12 respiratory viruses by using multiplex PCR technology and application thereof

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Embodiment Construction

[0087] Step 1. By analyzing the genome information of 12 kinds of respiratory viruses released by NCBI, the genes of each respiratory virus with high specificity and good conservation are obtained as target genes:

[0088] 1) Through the genome information of 12 kinds of viruses that NCBI has released, find the different genome sequences of each type of virus; then use DNAMAN software to perform genome sequence comparison on genome sequences, where figure 1 It is the result of genome sequence comparison analysis of the new coronavirus 2019-ncoV.

[0089] 2) Genome-specific genes were retrieved and compared with the nt database by blast software to determine specific gene sequences that did not have homology with the NCBI library as candidate genes. These specific sequences were homologous to homologous viruses. Source is not higher than 60%.

[0090] 3) Search the candidate genes in the genome data of the target virus to determine the conservation of their upstream and downst...

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Abstract

The invention discloses a method for rapidly detecting 12 respiratory viruses by using a multiplex PCR technology and application of the method, and belongs to the technical field of gene engineering.In order to solve the problems of difficult biological classification and poor specificity of 12 respiratory viruses at present, genomic information of 12 respiratory viruses published by NCBI is analyzed to obtain genes of each respiratory virus with high specificity and good conservative property as target genes, and multiple primers and probes are designed according to the target genes, a multiplex PCR reaction system is obtained through optimization, the method capable of rapidly detecting the 12 respiratory viruses is obtained, and whether a sample contains the 12 respiratory viruses ornot can be rapidly detected through one-time PCR by means of the method.

Description

technical field [0001] The invention belongs to the field of genetic engineering, and in particular relates to a method for rapidly detecting 12 kinds of respiratory viruses by using multiplex PCR technology and an application thereof. Background technique [0002] Infectious diseases are a type of diseases that the International Health Organization, major hospitals, and disease control centers are always on high alert for. They can be infected and transmitted between humans, animals, and humans. The outbreak and prevalence of infectious diseases pose a huge threat to human life and property. Infectious diseases can spread widely in cities around the world in a short period of time, seriously threatening human beings. Especially for diseases caused by respiratory viruses, how to efficiently identify suspected ones and achieve "early detection, early diagnosis, early isolation, and early treatment" has become a key means of virus infection treatment; especially the emergence ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/686C12N15/11
CPCC12Q1/701C12Q1/686C12Q2600/16C12Q2537/143C12Q2527/125
Inventor 刘志岩郭方刘珍郑青松
Owner 哈尔滨星云医学检验所有限公司
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