Probiotic culture medium and probiotic preparation

The technology of probiotics preparation and culture medium is applied in the field of probiotics culture medium and probiotics preparations, which can solve the problems of nitrogen consumption, complicated structure of a culture device, etc., and achieve the effect of promoting reproduction

Pending Publication Date: 2021-03-26
邢箫
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to make up for the deficiencies of the prior art, solve the problem that in the prior art, with the increase of metabolites of bifidobacteria during the cultivation process, the culture medium gradually tends to be acidic, thereby inhibiting the growth and reproduction of bifidobacteria. At the same time, the existing double-click bacillus cultivation device The structure is relatively complicated, and the problem of consuming more nitrogen in the process of use, a kind of probiotic culture medium and probiotic preparation proposed by the present invention

Method used

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  • Probiotic culture medium and probiotic preparation
  • Probiotic culture medium and probiotic preparation
  • Probiotic culture medium and probiotic preparation

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preparation example Construction

[0034] The preparation and application method of the probiotic culture medium comprise the following steps:

[0035] S1: Dissolve glucose, lactose, skimmed milk powder, soybean peptone, betaine and Tween-80 in deionized water. After the dissolution is completed, add sodium hydroxide solution dropwise to the mixture to adjust the pH value to 6.5-7.0 to obtain a probiotic Bacteria culture medium; use glucose, lactose as carbon source, skim milk powder to provide high-quality protein, and cooperate with soybean peptone to provide nitrogen source to provide sufficient nutrients for the growth of bifidobacteria. The addition of betaine and Tween-80 effectively cooperates Sodium hydroxide solution adjusts the pH value of the medium, and provides inorganic salt components for the growth and reproduction of bifidobacteria, and effectively enhances the survival rate of bifidobacteria during the freezing process of bifidobacteria;

[0036] S2: Add the adjusted probiotic culture medium i...

Embodiment approach

[0043] As an embodiment of the present invention, the bottom of the culture tray 2 is provided with a second cavity; the second cavity is connected to the first groove through a one-way conduit; the second cavity extends to Turn the side wall of the rotating disc, and open an air filling port on the side wall of the rotating disc; since the first grooves are evenly distributed on the culture disc 2, it is troublesome to replace the nitrogen gas one by one. When working, connect the nitrogen generating device with the second cavity , and then nitrogen is filled in the second cavity, when the petri dish 3 is filled in the conduction groove, close the sealing cover 25 immediately, and rotate the rotating plate 23, so that the rotating plate 23 squeezes the petri dish 3, and the petri dish 3. Squeeze completely into the first groove. Since the sliding plate 26 is completely sealed with the first groove, a negative pressure is formed above the sliding plate 26 during the downward sl...

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Abstract

The invention belongs to the technical field of microbial culture and particularly relates to a probiotic culture medium and a probiotic preparation. A culture rack comprises a base, culture disks andculture dishes; the base is designed to be T-shaped and a first cavity is formed in the base; the first cavity is filled with an aqueous solution; a temperature control system is arranged in the first cavity; the temperature control system is used for controlling a temperature of the aqueous solution; the culture disks are plural; connecting rods are fixedly connected to upper surfaces of the culture disks; connecting grooves are formed in surfaces of one sides, close to the base, of the culture disks; the connecting grooves penetrate through the culture disks and extend into the connecting rods; and the connecting grooves are designed in a stepped manner. By collecting and releasing carbon dioxide gas, an oxygen-free environment is provided for growth and reproduction of bifidobacteria,by means of an impact effect during release of carbon dioxide, a situation that external air, dust and impurities enter the culture dishes to cause negative influences on growth of the bifidobacteriais effectively avoided.

Description

technical field [0001] The invention belongs to the technical field of microorganism cultivation, in particular to a probiotic culture medium and a probiotic preparation. Background technique [0002] Since bifidobacteria are strict anaerobic bacteria, an anaerobic environment needs to be created during the cultivation process to facilitate the growth and reproduction of bifidobacteria. In the prior art, anaerobic bottles are often used to remove oxygen by passing nitrogen to create an anaerobic environment. However, during the growth of bifidobacteria, it is necessary to add alkaline substances to neutralize the culture medium many times, and then the anaerobic bottle is opened and closed many times. During this process, it is very easy for the outside air to enter together with dust and impurities. Inside the bottle, the culture medium will be polluted, and the bifidobacteria will be mixed with miscellaneous bacteria. At the same time, using anaerobic bottles for cultivati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12M1/22C12M1/00C12R1/01
CPCC12M23/10C12M23/36C12N1/20
Inventor 邢箫王乐
Owner 邢箫
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