Method for extracting soybean protein isolate from soybean whey wastewater
A technology for soybean whey wastewater and soybean protein isolate, which is used in the preparation methods of peptides, chemical instruments and methods, organic chemistry, etc.
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Embodiment 1
[0032] Step 1: pH adjustment: Take 41.9 kg of soybean whey wastewater generated during the production of soybean protein isolate, add sodium hydroxide solution, adjust the pH value to 7.0, and obtain soybean whey wastewater after adjusting the pH value;
[0033] Step 2: heating soybean whey wastewater: heating the soybean whey wastewater after adjusting the pH value in step 1, and heating to 70° C. with a heat exchanger;
[0034] Step 3: Membrane filtration: Filter the soybean whey wastewater heated in step 2 with a ceramic membrane, the pore size of the ceramic membrane is 0.1 μm, the working pressure of the ceramic membrane is 2.2 bar, the working temperature is 70°C, and the transmembrane pressure The difference (TMP) is 1.5bar, and the flux of the ceramic membrane is 312L / (m 2 * h), to obtain the intercepted concentrated solution;
[0035] Step 4: Dialysis: add the concentrated solution intercepted in step 3 into pure water for dialysis, remove residual salt, whey protein...
Embodiment 2
[0038] Step 1: pH adjustment: Take 125 kg of soybean whey wastewater generated during the production of soybean protein isolate, add sodium hydroxide solution, adjust the pH value to 7.5, and obtain soybean whey wastewater after adjusting the pH value;
[0039] Step 2: heating soybean whey wastewater: heating the soybean whey wastewater after adjusting the pH value in step 1, and heating to 75° C. with a heat exchanger;
[0040] Step 3: Microfiltration: Filter the soybean whey wastewater heated in step 2 with a ceramic membrane, the pore size of the ceramic membrane is 0.1 μm, the working pressure of the ceramic membrane is 2.5 bar, the working temperature is 75°C, and the transmembrane pressure The difference (TMP) is 1.8bar, and the flux of the ceramic membrane is 261L / (m 2 * h), to obtain the intercepted concentrated solution;
[0041] Step 4: Dialysis: add the concentrated solution intercepted in step 3 into pure water for dialysis, remove residual salt, whey protein, oli...
Embodiment 3
[0044] Step 1: pH adjustment: Take 441.76 kg of soybean whey wastewater in the process of extracting soybean protein isolate, add sodium hydroxide solution, adjust the pH value to 7.0, and obtain the soybean whey wastewater after adjusting the pH value;
[0045] Step 2: heating soybean whey wastewater: heating the soybean whey wastewater after adjusting the pH value in step 1, and heating to 80° C. with a heat exchanger;
[0046] Step 3: Microfiltration: Filter the soybean whey wastewater heated in step 2 with a ceramic membrane, the pore size of the ceramic membrane is 0.1 μm, the working pressure of the ceramic membrane is 2.4bar, the working temperature is 80°C, and the transmembrane pressure The difference (TMP) is 1.6bar, and the maximum flux of the ceramic membrane is 350L / (m 2 *h), to obtain the intercepted concentrated solution;
[0047] Step 4: Dialysis: add the concentrated solution intercepted in step 3 into pure water for dialysis, remove residual salt, whey prote...
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